PerCP anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
30-F11_PerCP_011508
C57BL/6 mouse splenocytes stained with 30-F11 PerCP
  • 30-F11_PerCP_011508
    C57BL/6 mouse splenocytes stained with 30-F11 PerCP
Compare all formats See PerCP spectral data
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103129 25 µg $107
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103130 100 µg $292
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Quispe Calla N, et al. 2016. Sci Rep. 6:37723. PubMed
  2. Filipello F et al. 2018. Immunity. 48(5):979-991 . PubMed
  3. Haratani K, et al. 2019. J Clin Invest. 130:374. PubMed
  4. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  5. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  6. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  7. Sun J, et al. 2020. Acta Biomater. 289:106. PubMed
  8. Shin J, et al. 2016. Sci Rep. 6:23426. PubMed
  9. Bo Yu et al. 2018. Cell stem cell. 23(2):193-209 . PubMed
  10. Keeter WC, et al. 2022. Eur Heart J Open. 2:oeac028. PubMed
  11. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
  12. Zhou J, et al. 2021. Int J Med Sci. 18:3516. PubMed
  13. Lopez-Guadamillas E, et al. 2016. Sci Rep. 6:34542. PubMed
  14. Kawashima N, et al. 2022. Nat Commun. 13:1624. PubMed
  15. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  16. Stolp B, et al. 2022. Cell Rep. 38:110387. PubMed
  17. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
  18. Tajfirouz D, et al. 2017. Virology. 10.1016/j.virol.2017.02.022. PubMed
  19. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  20. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  21. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  22. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  23. Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed
  24. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  25. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
  26. Liu YJ, et al. 2020. Theranostics. 10:5225. PubMed
  27. Nguyen CM, et al. 2019. Diabetes. 68:1499. PubMed
  28. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
  29. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  30. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  31. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
  32. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  33. Law HL, et al. 2020. Front Pharmacol. 11:274. PubMed
  34. Sears SM, et al. 2020. Am J Physiol Renal Physiol. 319:F674. PubMed
  35. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  36. Yang S, et al. 2019. Nat Commun. 10:2782. PubMed
  37. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  38. Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
  39. Amo-Aparicio J, et al. 2021. Theranostics. 11:9805. PubMed
  40. Saitoh BY, et al. 2021. Brain Behav Immun. 95:362. PubMed
  41. Aebischer D, et al. 2014. PLoS One. 9:99297. PubMed
  42. Everard A, et al. 2014. Nat Commun. 5:5648. PubMed
  43. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  44. Demandt JAF, et al. 2021. Frontiers in Cell and Developmental Biology. 9:664258. PubMed
  45. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  46. Kumaran Satyanarayanan S, et al. 2019. Nat Commun. 10:3471. PubMed
  47. Zou J, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:983. PubMed
  48. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  49. Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed
  50. Osaka M, et al. 2016. Sci Rep. 6:21391. PubMed
  51. Davis M, et al. 2015. J Immunol. 194:2219. PubMed
  52. West D, et al. 2014. J Immunol. 192:1762. PubMed
  53. Wang F, et al. 2019. Cell Death Dis. 1.036111111. PubMed
  54. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  55. Arcangeli S, et al. 2022. J Clin Invest. 132:. PubMed
  56. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
  57. Oldrini B, et al. 2018. Nat Commun. 9:1466. PubMed
  58. Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
  59. She L, et al. 2019. J Immunol. 203:2520. PubMed
  60. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
  61. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  62. Wells KL, et al. 2020. Elife. 9:. PubMed
  63. Voss M, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L188 - L195. PubMed
  64. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  65. Rangaraju S, et al. 2018. Mol Neurodegener. 13:24. PubMed
  66. Bogie JFJ, et al. 2021. J Autoimmun. 124:102723. PubMed
  67. Kovacs M, et al. 2021. Acta Neuropathol Commun. 9:136. PubMed
  68. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  69. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
  70. Peñaloza HF, et al. 2018. Biochem Biophys Rep. 13:12. PubMed
  71. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  72. Taddio MF, et al. 2021. Mol Imaging Biol. 23:196. PubMed
  73. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
  74. Woods S, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 308:912. PubMed
  75. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  76. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
  77. Li X, et al. 2021. Cell Death Dis. 12:314. PubMed
  78. Gorina R, et al. 2014. J Immunol. 192:324. PubMed
  79. Kerkhofs D, et al. 2020. Theranostics. 7.022222222. PubMed
  80. Yamamuro T, et al. 2020. Nat Commun. 3.340277778. PubMed
  81. Gomzikova MO, et al. 2020. Pharmaceutics. 12:00. PubMed
  82. Enam S, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.07.013. PubMed
  83. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  84. Doni A, et al. 2021. Nat Commun. 12:3739. PubMed
  85. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  86. Yamasaki R, et al. 2014. J Exp Med. 211:1533. PubMed
  87. Wong L, et al. 2013. J Biol Chem. 288:35170. PubMed
  88. Yin X, et al. 2015. J Immunol. 194:4029. PubMed
  89. SL C, et al. 2016. JCI Insight. 1: 83116. PubMed
  90. Cao Z, et al. 2021. Molecular Cell. 81(17):3604-3622.e10. PubMed
  91. Westrich JA, et al. 2019. Oncogene. 38:7166. PubMed
  92. Liu Q, et al. 2021. Adv Mater. 33:e2102852. PubMed
  93. Sears SM, et al. 2022. Kidney360. 3:818. PubMed
  94. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  95. Bai C, et al. 2022. Exp Ther Med. 24:673. PubMed
  96. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  97. Chen R, et al. 2019. Mol Med Rep. 1.172916667. PubMed
  98. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  99. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  100. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
  101. Chuang LT, et al. 2021. Molecules. 26:. PubMed
RRID
AB_893343 (BioLegend Cat. No. 103129)
AB_893339 (BioLegend Cat. No. 103130)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD45 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD45 30-F11 FC
Biotin anti-mouse CD45 30-F11 FC
FITC anti-mouse CD45 30-F11 FC
PE anti-mouse CD45 30-F11 FC
PE/Cyanine5 anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 30-F11 FC, IHC-F, CyTOF®, IP, CMCD, IHC, WB
PE/Cyanine7 anti-mouse CD45 30-F11 FC
APC/Cyanine7 anti-mouse CD45 30-F11 FC
Alexa Fluor® 488 anti-mouse CD45 30-F11 FC, SB
Alexa Fluor® 647 anti-mouse CD45 30-F11 FC, ICC, IHC, 3D IHC, SB
Pacific Blue™ anti-mouse CD45 30-F11 FC
Alexa Fluor® 700 anti-mouse CD45 30-F11 FC, SB
PerCP/Cyanine5.5 anti-mouse CD45 30-F11 FC
PerCP anti-mouse CD45 30-F11 FC
Alexa Fluor® 594 anti-mouse CD45 30-F11 IHC-F, FC, 3D IHC
Brilliant Violet 421™ anti-mouse CD45 30-F11 FC, SB
Brilliant Violet 570™ anti-mouse CD45 30-F11 FC
Brilliant Violet 510™ anti-mouse CD45 30-F11 FC
Brilliant Violet 605™ anti-mouse CD45 30-F11 FC
Purified anti-mouse CD45 (Maxpar® Ready) 30-F11 FC, CyTOF®
PE/Dazzle™ 594 anti-mouse CD45 30-F11 FC
Brilliant Violet 711™ anti-mouse CD45 30-F11 FC
Brilliant Violet 785™ anti-mouse CD45 30-F11 FC
Brilliant Violet 650™ anti-mouse CD45 30-F11 FC
APC/Fire™ 750 anti-mouse CD45 30-F11 FC
Brilliant Violet 750™ anti-mouse CD45 30-F11 FC
TotalSeq™-A0096 anti-mouse CD45 30-F11 PG
TotalSeq™-B0096 anti-mouse CD45 30-F11 PG
Ultra-LEAF™ Purified anti-mouse CD45 30-F11 FC, CyTOF®, IP, CMCD, IHC, WB
Spark Blue™ 550 anti-mouse CD45 30-F11 FC
Spark NIR™ 685 anti-mouse CD45 30-F11 FC
TotalSeq™-C0096 anti-mouse CD45 30-F11 PG
Spark YG™ 570 anti-mouse CD45 30-F11 IHC-F
PE/Fire™ 640 anti-mouse CD45 30-F11 FC
APC/Fire™ 810 anti-mouse CD45 30-F11 FC
PE/Fire™ 700 anti-mouse CD45 30-F11 FC
Spark Violet™ 538 anti-mouse CD45 30-F11 FC
Spark YG™ 593 anti-mouse CD45 30-F11 FC
Spark Blue™ 574 anti-mouse CD45 Antibody 30-F11 FC
Go To Top Version: 2    Revision Date: 05/14/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD45

  • Biotin anti-mouse CD45

  • FITC anti-mouse CD45

  • PE anti-mouse CD45

  • PE/Cyanine5 anti-mouse CD45

  • Purified anti-mouse CD45

  • PE/Cyanine7 anti-mouse CD45

  • APC/Cyanine7 anti-mouse CD45

  • Alexa Fluor® 488 anti-mouse CD45

  • Alexa Fluor® 647 anti-mouse CD45

  • Pacific Blue™ anti-mouse CD45

  • Alexa Fluor® 700 anti-mouse CD45

  • PerCP/Cyanine5.5 anti-mouse CD45

  • PerCP anti-mouse CD45

  • Alexa Fluor® 594 anti-mouse CD45

  • Brilliant Violet 421™ anti-mouse CD45

  • Brilliant Violet 570™ anti-mouse CD45

  • Brilliant Violet 510™ anti-mouse CD45

  • Brilliant Violet 605™ anti-mouse CD45

  • Purified anti-mouse CD45 (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse CD45

  • Brilliant Violet 711™ anti-mouse CD45

  • Brilliant Violet 785™ anti-mouse CD45

  • Brilliant Violet 650™ anti-mouse CD45

  • APC/Fire™ 750 anti-mouse CD45

  • Brilliant Violet 750™ anti-mouse CD45

  • TotalSeq™-A0096 anti-mouse CD45

  • TotalSeq™-B0096 anti-mouse CD45

  • Ultra-LEAF™ Purified anti-mouse CD45

  • Spark Blue™ 550 anti-mouse CD45

  • Spark NIR™ 685 anti-mouse CD45

  • TotalSeq™-C0096 anti-mouse CD45

  • Spark YG™ 570 anti-mouse CD45

  • PE/Fire™ 640 anti-mouse CD45

  • APC/Fire™ 810 anti-mouse CD45

  • PE/Fire™ 700 anti-mouse CD45

  • Spark Violet™ 538 anti-mouse CD45

  • Spark YG™ 593 anti-mouse CD45

  • Spark Blue™ 574 anti-mouse CD45 Antibody

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