PerCP anti-human CD27 Antibody

Pricing & Availability
Clone
M-T271 (See other available formats)
Workshop
V 5T CD27.03
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M-T271_PerCP_CD27_Antibody_FC_011017
Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) PerCP (filled histogram) or mouse IgG1, κ isotype control (open histogram).
  • M-T271_PerCP_CD27_Antibody_FC_011017
    Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) PerCP (filled histogram) or mouse IgG1, κ isotype control (open histogram).
See PerCP spectral data
Cat # Size Price Quantity Avail. Save
356431 25 tests $130
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356432 100 tests $285
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reacitivity: Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human T cells from a T-ALL patient.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections1, immunofluorescent staining2, and ELISA3.

Application References

(PubMed link indicates BioLegend citation)
  1. Ma S, et al. 2011. J. Virol. 85:165. (IHC)
  2. Manzo A, et al. 2008. Arthritis Rheum. 11:3377. (IF)
  3. Kato K, et al. 2007. Exp. Hematol. 35:434. (ELISA)
RRID
AB_2650752 (BioLegend Cat. No. 356431)
AB_2650753 (BioLegend Cat. No. 356432)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution

Medullary thymocytes, T and B cell subsets, NK cells

Interaction
CD27 binds to CD70
Ligand Receptor
CD70
Cell Type
Thymocytes, T cells, B cells, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Hintzen R, et al. 1994. Immunol. Today 15:307.
4. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Go To Top Version: 1    Revision Date: 01/11/2017

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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