PE/Dazzle™ 594 anti-mouse H-2Kb bound to SIINFEKL Antibody

Pricing & Availability
Clone
25-D1.16 (See other available formats)
Other Names
MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
25-D116_PEDazzle594_H-2kb_Antibody_092718
C57BL/6 mouse splenocytes were pulsed with or without SIINFEKL for 2 hours, and then stained with anti-mouse SIINFEKL bound H-2Kb (clone 25-D1.16) PE/Dazzle™ 594 (purple filled histogram indicates the pulsed cells and cyan open histogram indicates non-pulsed cells) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (black open histogram).
  • 25-D116_PEDazzle594_H-2kb_Antibody_092718
    C57BL/6 mouse splenocytes were pulsed with or without SIINFEKL for 2 hours, and then stained with anti-mouse SIINFEKL bound H-2Kb (clone 25-D1.16) PE/Dazzle™ 594 (purple filled histogram indicates the pulsed cells and cyan open histogram indicates non-pulsed cells) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (black open histogram).
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Avail. Save
141611 25 µg $110
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141612 100 µg $325
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Description

This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SIINFEKL pulsed RMA-S cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide.  Additional reported applications (for relevant formats) include: Western Blotting1,3, immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.

Application References

(PubMed link indicates BioLegend citation)
  1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78. (WB)
  2. Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF)
  3. Porgador A, et al. 1997. Immunity 6:715. (WB, IF, IHC)
  4. Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.
RRID
AB_2750521 (BioLegend Cat. No. 141611)
AB_2750522 (BioLegend Cat. No. 141612)

Antigen Details

Structure
OVA peptide257-264 and H-2Kb complex
Distribution

SIINFEKL bond H-2Kb bearing cells

Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78.
2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053.
3. Deng Y, et al. 1998. J. Immunol. 161:1677.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kb bound to SIINFEKL on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/28/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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