PE/Dazzle™ 594 anti-human HLA-E Antibody

Pricing & Availability
Clone
3D12 (See other available formats)
Other Names
HLA class I histocompatibility antigen, alpha chain E, HLAE
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3D12_PEDazzle594_HLA-E_Antibody_070219
Human peripheral blood lymphocytes were stained with anti-human HLA-E (clone 3D12) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
  • 3D12_PEDazzle594_HLA-E_Antibody_070219
    Human peripheral blood lymphocytes were stained with anti-human HLA-E (clone 3D12) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Avail. Save
342615 25 tests $120
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342616 100 tests $310
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Description

HLA-E is a non-classical MHC class I (MHC-Ib) molecule. It is characterized by limited polymorphism and ubiquitous expression. HLA-E, as other MHC-I molecules, is heterodimerized with β2-microglobulin. HLA-E interacts with CD94/NKG2 receptor to regulate NK cell cytolysis activities.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Lee N, et al. 1998. Proc. Natl. Acad. Sci. USA. 95:5199.
  2. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  3. Monaco EL, et al. 2008. J. Immunol. 181:5442.
  4. Corrah TW, et al. 2011. J. Virol. 85:3367. PubMed

Antigen Details

Structure
HLA family, Ig superfamily, 42 kD
Distribution

Broadly expressed

Function
Mediate cell recognition by NK cells
Ligand/Receptor
CD94/NKG2
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References

1. Sullivan LC, et al. 2008. Tissue Antigen. 72:415.
2. Koller BH, et al. 1988. J. Immunol. 141:897.

Gene ID
3133 View all products for this Gene ID
UniProt
View information about HLA-E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/02/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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