PE/Cy7 anti-human CD50 (ICAM-3) Antibody

Pricing & Availability
Clone
CBR-IC3/1 (See other available formats)
Other Names
ICAM-3
Isotype
Mouse IgG1, κ
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Product Citations
publications
CBR-IC3slash1_PECy7_CD50_Antibody_021918
Human peripheral blood lymphocytes were stained with anti-human CD50 (ICAM-3) (clone CBR-IC3/1) PE/Cy7 (closed histogram) or mouse IgG1, κ PE/Cy7 isotype control (open histogram).
  • CBR-IC3slash1_PECy7_CD50_Antibody_021918
    Human peripheral blood lymphocytes were stained with anti-human CD50 (ICAM-3) (clone CBR-IC3/1) PE/Cy7 (closed histogram) or mouse IgG1, κ PE/Cy7 isotype control (open histogram).
See PE/Cy7 spectral data
Cat # Size Price Quantity Avail. Save
330015 25 tests $135
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330016 100 tests $315
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Description

CD50 is a 120-140 kD type I membrane protein also known as intercellular adhesion molecule 3 (ICAM-3). It is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. CD50 is a member of the Ig superfamily. It provides costimulatory signal for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a/CD18, αL2) integrin.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SKW3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunoprecipitation1 and blocking1-3.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. deFougerolles AR and Springer TA, et al. 1992. J. Exp. Med. 175:185. (IP, Block)
  2. Geijtenbeek T, et al. 2000. Cell 100:575. (Block)
  3. Giannoni E, et al. 2003. J Biol. Chem. 278:36763. (Block)
RRID
AB_2728277 (BioLegend Cat. No. 330015)
AB_2728278 (BioLegend Cat. No. 330016)

Antigen Details

Structure
Ig superfamily, type I membrane protein, 130 kD
Distribution

Thymocytes, T and B cells, monocytes, granulocytes, endothelial cells

Function
Adhesion, costimulation
Ligand/Receptor
CD11a/CD18 (LFA-1), integrin αL2
Cell Type
Thymocytes, T cells, B cells, Monocytes, Granulocytes, Endothelial cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Valent P, et al. 2001. Immunol. Rev. 179:74.

Gene ID
3385 View all products for this Gene ID
UniProt
View information about CD50 (ICAM-3) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/20/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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