PE/Dazzle™ 594 anti-mouse CD226 (DNAM-1) Antibody

Pricing & Availability
Clone
TX42.1 (See other available formats)
Other Names
DNAM-1, PTA1 (Platelet and T cell activation antigen 1), TLISA1, LFA-1 associated molecule PTA-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
TX42dot1_PEDazzle594_CD226_Antibody_1_082417
C57BL/6 mouse splenocytes were stained with CD8 FITC and CD226 (clone TX42.1) PE/Dazzle™ 594 (top) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (bottom).
  • TX42dot1_PEDazzle594_CD226_Antibody_1_082417
    C57BL/6 mouse splenocytes were stained with CD8 FITC and CD226 (clone TX42.1) PE/Dazzle™ 594 (top) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (bottom).
  • TX42dot1_PEDazzle594_CD226_Antibody_2_082417
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Avail. Save
133617 25 µg $150
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133618 100 µg $325
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Description

Mouse CD226 (DNAM-1) is a 65 kd glycoprotein of a member of Ig super-family adhesion molecule containing 2 immunoglobulin-like domains. It is associated with leukocyte function associated antigen-1 (LFA-1) involving in TCR-mediated signal transduction for T cell proliferation and differentiation. CD226 is also involved in NK cell and T cell mediated cytotoxicity against certain tumors. CD226 (DNAM-1) is constitutively expressed on naïve CD8+ and CD4+ T cells, macrophages, and NK cells. It was reported that CD226 expression is up-regulated on Th1 cells, but down-regulated on Th2 and Th0 cells. CD155 and CD112 are the ligands for CD226.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse DNAM-1 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Dardalhon V, et al. 2005. J. Immunol. 175:1558
  2. Shirakawa J, et al. 2006. International Immunology 18(6):951-957
RRID
AB_2716079 (BioLegend Cat. No. 133617)
AB_2716080 (BioLegend Cat. No. 133618)

Antigen Details

Structure
65 kd glycoprotein of a member of Ig super-family containing 2 immunoglobulin-like domains.
Distribution

Expressed on majority of CD8+ and CD4+ T cells, NK cells, monocytes/macrophages and a subset of B cells. Reported to up-regulated on Th1 cells, but down-regulated in Th2 cells.

Function
Mediate cellular adhesion, involved in LFA-1 co-stimulatory signal for T cell differentiation and proliferation.
Ligand/Receptor
CD112 and CD155
Cell Type
T cells, NK cells, Monocytes, Macrophages, Th1, B cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1 Tahara Hanaoka S, et al. 2006. Blood. 107:1491
2 Shibuya K et al. 2003. J. Exp. Med. 198:1829.

Gene ID
225825 View all products for this Gene ID
UniProt
View information about CD226 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 08/25/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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