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PE/Cyanine7 anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
11-26C.2A_PECy7_IgD_Antibody_FC_1_021913
C57BL/6 splenocytes were stained with CD45R/B220 FITC and IgD (clone 11-26c.2a) PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom).
  • 11-26C.2A_PECy7_IgD_Antibody_FC_1_021913
    C57BL/6 splenocytes were stained with CD45R/B220 FITC and IgD (clone 11-26c.2a) PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom).
  • 11-26C.2A_PECy7_IgD_Antibody_FC_2_021913
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
405719 25 µg $129
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405720 100 µg $340
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Description

Surface IgD is an important B cell differentiation marker.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed
  2. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  3. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  4. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  5. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  6. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  7. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  8. Werner A, et al. 2021. iScience. 24:103076. PubMed
  9. Escolano A, et al. 2016. Cell. 166(6):1445-1458.e12. PubMed
  10. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  11. Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
RRID
AB_2561875 (BioLegend Cat. No. 405719)
AB_2561876 (BioLegend Cat. No. 405720)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/20/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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