PE/Cyanine7 anti-mouse/human CD282 (TLR2) Recombinant antibody, CD282, QA16A01

Pricing & Availability

Clone: QA16A01 (See other available formats)
Regulatory Status: RUO
Other Names: Toll-like receptor 2, TLR-2, TIL4, CD282, TLR2
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood cells were stained with PE/Cyanine7 anti-mouse/human CD282 (TLR2) recombinant (clone QA16A01) (filled histogram) or isotype control mouse IgG1, κ PE/Cyanine7 (open histogram).

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153011

25 µg

$176

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Description

CD282 (Toll-like receptor 2, TLR2) is an 85kDa, type I transmembrane protein, with 14 leucine-rich repeats (LRR) in the extracellular domain and a toll/IL-1R (TIR) in the cytoplasmic region. TLR2 is expressed by monocytes/macrophages, dendritic cells, granulocytes, endothelial cell and epithelial cells. TLR2 associates with TLR1 and TLR6 in the cell surface and is involved in the recognition of pathogen-associated molecular patterns (PAMPs) such as peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, and LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse, Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Recombinant extracellular domain of murine TLR2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Blocking of IL-6 and TNF-a production induced by P₃CSK₄ on RAW264.7 cells was observed during development.
RRID: AB_2894693 (BioLegend Cat. No. 153011)

Antigen Details

Structure: Type I transmembrane protein, 14 leucine-rich repeats (LRR) in the extracellular domain, the intracellular domain contains a toll/IL-1R (TIR) domain.
Distribution: Monocytes/macrophages, dendritic cells, granulocytes, endothelial cell, epithelial cells.
Function: Activates innate host defense, induces production of pro-inflammatory cytokines.
Interaction: TLR1, TLR6 , MYD88 and TRAF6.
Ligand/Receptor: Peptidoglycan, lipoteihoic acid (LTA), lipoarabinomanan, lipoproteins, as well as different LPS from certain Gram-negative bacteria, yeast, spirochete and fungi.
Cell Type: Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: CD Molecules, Toll Like Receptors
Antigen References: 1. Biswas S, et al. 2016. Blood. 127:2618.
2. Wang Y, et al. 2016. Proc. Natl. Acad. Sci. USA 113:884.
3. Ramstead AG, et al. 2016. Infect. Immun. 84:940.
4. Scheeren FA, et al. 2014. Nat. Cell. Biol. 16:1238.
5. Barbalat R, et al. 2009. Nat. Immunol. 10:1200.
Gene ID: 24088 View all products for this Gene ID 7097 View all products for this Gene ID
UniProt: View information about CD282 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD282 Reagents Request Custom Conjugation

Description	Clone	Applications
Ultra-LEAF™ Purified anti-mouse/human CD282 (TLR2) Recombinant Antibody	QA16A01	FC,Block
APC anti-mouse/human CD282 (TLR2) Recombinant Antibody	QA16A01	FC
Alexa Fluor® 647 anti-mouse/human CD282 (TLR2) Recombinant Antibody	QA16A01	FC
PE anti-mouse/human CD282 (TLR2) Recombinant Antibody	QA16A01	FC
KIRAVIA Blue 520™ anti-mouse/human CD282 (TLR2) Recombinant Antibody	QA16A01	FC
PE/Cyanine7 anti-mouse/human CD282 (TLR2) Recombinant Antibody	QA16A01	FC

Concentration & Expiration Lookup

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06/30/2021

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