PE/Cyanine7 anti-mouse CD8b.2 Antibody

Pricing & Availability
53-5.8 (See other available formats)
Regulatory Status
Other Names
Lyt-3.2, Ly-3.2
Rat IgG1, κ
Ave. Rating
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Product Citations
C57BL/6 mouse splenocytes were stained with CD8a Alexa Fluor® 488 CD8b.2 (clone 53-5.8) PE/Cyanine7 (top) or rat IgG1, κ PE/Cyanine7 isotype control (bottom).
  • 53-5.8_PeCy7_CD8b.2_FC_1_101314
    C57BL/6 mouse splenocytes were stained with CD8a Alexa Fluor® 488 CD8b.2 (clone 53-5.8) PE/Cyanine7 (top) or rat IgG1, κ PE/Cyanine7 isotype control (bottom).
  • 53-5.8_PeCy7_CD8b.2_FC_2_101314
Compare all formats See PE/Cyanine7 spectral data
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140415 25 µg $159
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140416 100 µg $347
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CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse thymus or spleen
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter J, et al. 1979. Immunol. Rev. 47:63.
  2. Ledbetter J, et al. 1980. J. Exp. Med. 152:280.
  3. Vremec D, et al. 2000. J. Immunol. 164:2978. (IF)
  4. Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC)
  5. Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
Product Citations
  1. Lu SX, et al. 2021. Cell. . PubMed
  2. Zhou Y, et al. 2020. Immunity. 52(2):357-373. PubMed
  3. Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed
  4. Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed
  5. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  6. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
AB_2564384 (BioLegend Cat. No. 140415)
AB_2564385 (BioLegend Cat. No. 140416)

Antigen Details

Ig superfamily, CD8β chain, 32 kD

Majority of thymocytes, cytotoxic T cell subsets

Co-receptor of TCR
MHC class I
Cell Type
T cells, Thymocytes
Biology Area
Molecular Family
CD Molecules, TCRs
Antigen References

1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503.
2. Renard V, et al. 1996. J. Exp. Med. 184:2439.

Gene ID
12526 View all products for this Gene ID
View information about CD8b.2 on

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Go To Top Version: 1    Revision Date: 10/20/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD8b.2

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  • Biotin anti-mouse CD8b.2

  • PE anti-mouse CD8b.2

  • APC anti-mouse CD8b.2

  • Pacific Blue™ anti-mouse CD8b.2

  • PE/Cyanine7 anti-mouse CD8b.2

  • PerCP/Cyanine5.5 anti-mouse CD8b.2

  • APC/Fire™ 750 anti-mouse CD8b.2

  • APC/Cyanine7 anti-mouse CD8b.2


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