PE/Cyanine7 anti-mouse CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A7R34 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_A7R34_PECy7_110609
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and A7R34 PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom)
  • A_A7R34_PECy7_110609
    C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and A7R34 PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom)
  • B_A7R34_PECy7_110609
See PE/Cyanine7 spectral data
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135013 25 µg $105.00
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135014 100 µg $320.00
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-7Ra-IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

A7R34 is able to block clone SB/199 binding to IL-7R.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
  2. Hashi H, et al. 2001. J. Immunol. 166:3702.
  3. Taylor R, et al. 2007. J. Immunol. 178:5659.
  4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
  5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations
  1. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  2. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  3. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  4. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  5. Page N, et al. 2018. Immunity. 48:937. PubMed
  6. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  7. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  8. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  9. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  10. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  11. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  12. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  13. Zheng X, et al. 2019. PLoS Pathog. 15:e1008036. PubMed
  14. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  15. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  16. Olguín JE, et al. 2018. J Cancer. 9:239. PubMed
  17. Pokrovskii M, et al. 2019. Immunity. 51:185. PubMed
  18. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  19. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
  20. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  21. Zhong C, et al. 2020. Immunity. 52(1):83-95.e4.. PubMed
  22. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  23. Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
  24. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  25. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  26. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
  27. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  28. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
RRID
AB_1937266 (BioLegend Cat. No. 135013)
AB_1937265 (BioLegend Cat. No. 135014)

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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