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PE/Cyanine7 anti-mouse CD117 (c-kit) Antibody

Pricing & Availability
Clone
ACK2 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_ACK2_PECy7_020210.jpg
Bone marrow cells from C57BL/6 mice stained with ACK2 PE/Cyanine7 and CD11b (M1/70) APC
  • A_ACK2_PECy7_020210.jpg
    Bone marrow cells from C57BL/6 mice stained with ACK2 PE/Cyanine7 and CD11b (M1/70) APC
  • B_ACK2_PECy7_020210.jpg
    Bone marrow cells from C57BL/6 mice stained with rat IgG2b PE/Cyanine7 isotype control and CD11b (M1/70) APC
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
135111 25 µg $72
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135112 100 µg $209
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Description

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine IL-3 dependent mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3

ACK2 has been reported to block c-Kit function. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Yoshinaga K, et al. 1991. Development 113:689.
  2. Broudy VC, et al. 1996. Blood 88:75.
  3. Louvet C, et al. 2008. Proc. Natl. Acad. Sci. USA 105:18895.
Product Citations
  1. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  2. Cho K, et al. 2021. iScience. 24:103117. PubMed
  3. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  4. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  5. Kitadate Y, et al. 2019. Cell Stem Cell. 24:79. PubMed
  6. Gong Y, et al. 2014. PLoS One. 9:95898. PubMed
  7. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
RRID
AB_2131136 (BioLegend Cat. No. 135111)
AB_2131135 (BioLegend Cat. No. 135112)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ. et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P et al. 1986. Nature 320:415.
5. Witte ON. et al. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/15/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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