PE/Cyanine5 anti-mouse CD43 Activation-Associated Glycoform Antibody

Pricing & Availability
1B11 (See other available formats)
Regulatory Status
Other Names
Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, gp115
Rat IgG2a, κ
Ave. Rating
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Product Citations
C57BL/6 mouse splenocyts stained with 1B11 PE/Cyanine5
  • 1B11_PECy5_042408
    C57BL/6 mouse splenocyts stained with 1B11 PE/Cyanine5
Compare all formats See PE/Cyanine5 spectral data
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121216 100 µg $305
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CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse WEHI 274.3 myeloid tumor cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, and immunohistochemistry of acetone-fixed frozen sections.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
  2. Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
  3. Onami TM, et al. 2002. J. Immunol. 168:6022.
  4. van der Most RG, et al. 2003. Intl. Immunol. 15:119.
  5. Chu VT, et al. 2007. J. Immunol. 179:5947.
  6. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
  7. Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed
Product Citations
  1. Hill B, et al. 2014. J Immunol. 193:5626. PubMed
AB_528811 (BioLegend Cat. No. 121216)

Antigen Details

Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation

Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5+ B-1 cells and activated B cells, and hemotopoietic progenitor cells.

Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
CD54, Siglec-1
Cell Type
B cells, Granulocytes, Hematopoietic stem and progenitors, Monocytes, NK cells, Plasma cells, Platelets, T cells, Thymocytes
Biology Area
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. van den Berg TK, et al. 2001. J. Immunol. 166:3637.
2. Moore T, et al. 1994. J. Immunol. 153:4978.
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. Tong J, et al. 2004. J. Exp. Med. 199:1277.
5. Jones AT, et al. 1994. J. Immunol. 153:3426.

Gene ID
20737 View all products for this Gene ID
View information about CD43 on

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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