PE/Cy7 anti-human CD55 Antibody

Pricing & Availability
Clone
JS11 (See other available formats)
Workshop
VI N-L060
Other Names
DAF (Decay Accelerating Factor)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
JS11_PECy7_022508
Human peripheral blood lymphocytes stained with JS11 PE/Cy7
  • JS11_PECy7_022508
    Human peripheral blood lymphocytes stained with JS11 PE/Cy7
See PE/Cy7 spectral data
Cat # Size Price Quantity Avail. Save
311314 100 tests $275
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Description

CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein also known as decay-accelerating factor (DAF). It is expressed on hematopoietic cells including erythrocytes and many non-hematopoietic cells. CD55 accelerates the dissociation of the components of the C3-convertases (namely C2a from C4b in the C4bC2a complex, a C3-convertase of the classical pathway, and factor Bb from the C3bBb complex, a C3-convertase of the alternative pathway) to protect cells from inappropriate damage caused by autologous complement. CD55 has been reported to reduce the efficiency of NK cell lysis and induce signal transduction in T cells. CD55 has also been shown to interact with CD97 and bind to Coxsackie and Echovirus.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Peyron P, et al. 2000. J. Immunol. 165:5186
Product Citations
  1. Sebejova L, et al. 2014. Exp Hematol. 42:867. PubMed
  2. Rennert R, et al. 2016. Nat Commun. 7:11945. PubMed
  3. Davies L, et al. 2016. Stem Cells Trans Med. 5: 1485 - 1495. PubMed
Publication Library
RRID
AB_2075855 (BioLegend Cat. No. 311314)

Antigen Details

Structure
GPI-achored regulator of complement activation (RCA), type I membrane glycoprotein, 60-70 kD
Distribution

Hematopoietic cells including erythrocytes and many non-hematopoietic cells

Function
Protects against inappropriate complement activation and cell damage, induces signal transduction, reduces the efficiency of NK cell lysis
Ligand/Receptor
C3b, C4b, CD97, Coxsackie viruses
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Hamann J, et al. 1996. J. Exp. Med. 184:1185.
3. Fujita T, et al. 1987. J. Exp. Med. 166:1221

Gene ID
1604 View all products for this Gene ID
UniProt
View information about CD55 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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