- 143F (See other available formats)
- Other Names
- X-box binding protein 1
- Mouse IgG2a
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- Product Citations
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XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of human HLA DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 upregulates as part of the ER stress response, also known as the unfolded protein response. Unspliced XBP-1 is 261 amino acids and migrates on the SDS-PAGE around 33 kD; spliced XBP-1, recognized by clone 143F, is 371 amino acids and migrates around 55 kD.Product Details
- Antibody Type
- Host Species
- XBP-1s recombinant protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence14 and immunohistochemical staining of PFA-fixed paraffin-embedded sections1,14.
(PubMed link indicates BioLegend citation)
- Tsang KY, et al. 2007. PLOS Biol. 5:568. (IHC) PubMed
- Farhan H, et al. 2008. EMBO J. 27:2043 (WB) PubMed
- Farhan H, et al. 2010. J. Cell Biol. 189:997. PubMed
- Feng-Jin G, et al. 2010. Cell Signal. [Epub ahead of print]
- Wang L, et al. 2011. Hum Mol Genet. PubMed
- Li J, et al. 2011. J. Biol Chem. 286:4912. PubMed
- Wang L, et al. 2011. Hum Mol Genet. 20:1008. PubMed
- Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed
- Shirley CM, et al. 2011 Blood. 117:6297. PubMed
- Vidal RL., et al. 2012. Hum Mol Genet. PubMed
- Byrd AE, et al. 2012. J Cell Biol. 196:689. PubMed
- Dickie LJ, et al. 2012. Ann rheum Dis. 71:2035. PubMed
- Bonetti P, et al. 2013. Blood. 122:2233. PubMed
- Maestre L, et al. 2009. Haematologica. 94:419. (IF, IHC)
- Rodriguez M, et al. 2014. J Biol Chem. 289:22942. PubMed
AB_2563447 (BioLegend Cat. No. 647503)
AB_2563448 (BioLegend Cat. No. 647504)
- Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
- Biology Area
- Cell Biology
- Antigen References
1. Liou HC, et al. 1990. Science 247:1581.
2. Ponath PD, et al. 1993. J. Biol. Chem.268:17074.
3. Takahashi S, et al. 2002. Prostate 50:154.
- Gene ID
- 7494 View all products for this Gene ID
- View information about XBP-1s on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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