- TRIM-4 (See other available formats)
- Regulatory Status
- Other Names
- T cell receptor-interacting molecule
- Mouse IgG2a, κ
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- Product Citations
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TRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit α after phosphorylation. TRIM has also been shown to associate with TCRα, β, and ζ. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Host Species
- Partial recombinant human TRIM (amino acids 29-186)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: intracellular flow cytometry1, ELISA1, and immunofluorescence microscopy2.
Clone TRIM-4 has been shown to cross-react to mouse TRIM1.
(PubMed link indicates BioLegend citation)
- Huynh T, et al. 2001. Scand. J. Immunol. 54:146.
- Kirchgessner H, et al. 2001. J. Exp. Med. 193:1269.
AB_2565761 (BioLegend Cat. No. 627203)
AB_2565762 (BioLegend Cat. No. 627204)
- Type III membrane protein, 30 kD disulfide-linked dimeric phosphoprotein
- T cells, lower expression levels in NK cells
- Links extracellular and intracellular signaling pathways in T cell activation
- PI3K regulatory subunit α, TCRα, β, and ζ.
- Tyrosine phosphorylation by Src kinases
- Cell Type
- T cells, NK cells
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Antigen References
1. Bruyns E, et al. 1998. J. Exp. Med. 188:561.
2. Hubener C, et al. 2000. Immunogenetics 51:154.
- Gene ID
- 50852 View all products for this Gene ID
- View information about TRIM on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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