PE anti-Siglec-E Antibody

Pricing & Availability
Clone
M1304A01 (See other available formats)
Other Names
Sialic acid-binding Ig-like lectin, Siglec E
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M1304A01_PE_Siglec-E_Antibody_FC_1_102715
C57BL/6 mouse splenocytes were stained with Ly-6G/Ly-6C (clone Gr-1) FITC and Siglec-E (clone M1304A01) PE (top) or rat IgG2a, κ PE isotype control (bottom).
  • M1304A01_PE_Siglec-E_Antibody_FC_1_102715
    C57BL/6 mouse splenocytes were stained with Ly-6G/Ly-6C (clone Gr-1) FITC and Siglec-E (clone M1304A01) PE (top) or rat IgG2a, κ PE isotype control (bottom).
  • M1304A01_PE_Siglec-E_Antibody_FC_2_102715
See PE spectral data
Cat # Size Price Quantity Avail. Save
677103 25 µg $125
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677104 100 µg $295
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Description

Siglecs (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region containing a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. The cytoplasmic tails of all siglecs have tyrosine based motifs with a signaling function. Siglecs are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Studies suggest that siglecs may participate in cell-cell interactions or act as receptors for the entry of viral or bacterial pathogens. In addition, the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domain indicates that these molecules may play a role in the suppression of immunoreceptor signaling. Siglec-E is a mouse CD33-related siglec that selectively regulates early recruitment of neutrophils to the lung in acute lung inflammation induced by lipopolysaccharide. Siglec E-deficient mice exhibit exaggerated neutrophil recruitment that is reversible by using a blockade of the β2 integrin, CD11b. In addition, sialidase treatment of fibrinogen reverses the suppressive effect of Siglec-E on CD11b signaling. This suggests that sialic acid recognition by Siglec-E is required for its inhibitory function. These findings indicate that Siglec-E is an important negative regulator of neutrophil recruitment to the lungs and β2 integrin-dependent signaling.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse Siglec-E produced in the HEK293A cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This antibody works for western blotting under non-reducing conditions.

Application References

(PubMed link indicates BioLegend citation)
  1. Siddiqui S, et. al. 2017. J. Biol. Chem. 292: 1029.
RRID
AB_2566168 (BioLegend Cat. No. 677103)
AB_2566169 (BioLegend Cat. No. 677104)

Antigen Details

Structure
569 amino acids with a predicted molecular weight of approximately 65 kD.
Distribution
Siglec-E is expressed mainly on neutrophils in blood and their immature precursors in bone marrow, on tissue macrophages, on subsets of mature natural killer cells, splenic dendritic cells, and on monocytes as well.
Function
Siglec-E regulates neutrophil recruitment. Siglec-E signaling negatively regulates the LPS-induced production of TNF-α and IL-6 by macrophages. It is upregulated by a variety of TLR agonists.
Ligand Receptor
Siglec-E binds broadly to sialic acid-containing glycans. It prefers alpha 2,8-linked sialic acid over alpha 2,6 and alpha 2,3.
Cell Type
Neutrophils, Macrophages, NK cells, Dendritic cells, Monocytes, B cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Siglec Molecules
Antigen References

1. McMillan SJ, et al. 2013. Blood 121:2084.
2. Bax M, et al. 2010. Ann. Rheum. Dis. 69:42.
3. Angata T and Varki A. 2000. J. Biol. Chem. 275:22127.
4. Zhang JQ, et al. 2004. Eur. J. Immunol. 34:1175.
5. Yu Z, et al. 2001. Biochem. J. 353:483.
6. Claude J, et al. 2013. J. Neurosci. 33:18270.
7. Chang YC, et al. 2014. PLoS Pathog. 10:e1003846.
8. Laubli H, et al. 2014. Proc. Natl. Acad. Sci. USA 111:14211.
9. Schwarz F, et al. 2015. eLife 4:e06184.

Gene ID
83382 View all products for this Gene ID
UniProt
View information about Siglec-E on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/08/2017

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