PE anti-p53 Antibody

Pricing & Availability
Clone
DO-7 (See other available formats)
Other Names
Tumor protein p53 (TP53)
Isotype
Mouse IgG2b
Ave. Rating
1 reviews
Product Citations
publications
DO-7_PE_p53_Antibody_FC_062513
A431 human breast cancer cell line was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with p53 (clone DO-7) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • DO-7_PE_p53_Antibody_FC_062513
    A431 human breast cancer cell line was fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with p53 (clone DO-7) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Avail. Save
645805 25 tests $120
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645806 100 tests $265
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Description

p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
p53 N-terminal linear epitope aa 20-25
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Vojtesek B, et al. 1992. J. Immunol. Methods 151:237.
  2. Stephen CW, et al. 1995. J. Mol. Biol. 248:58.
Product Citations
  1. Catenacci D, et al. 2015. Gastroenterology. 149: 1794-1803.e4. PubMed
Publication Library
RRID
AB_2562267 (BioLegend Cat. No. 645805)
AB_2562268 (BioLegend Cat. No. 645806)

Antigen Details

Structure
Tetramer; 53 kD
Distribution

Nuclear when activated

Function
Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
Interaction
mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, DNA Repair/Replication, Stem Cells
Gene ID
7157 View all products for this Gene ID
UniProt
View information about p53 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/17/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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