PE anti-mouse TNF-α Antibody

Pricing & Availability
Clone
MP6-XT22 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2, TNF-a, TNF-alpha
Isotype
Rat IgG1, κ
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Product Citations
publications
MP6-XT22_PE_TNF-a_Antibody_FC_1_100913
PMA + Ionomycin-stimulated C57BL/6 mouse splenocytes (in the presence of monensin) were stained with CD3 APC, fixed, permeabilized and then stained with TNF-α (clone MP6-XT22) PE (top) or rat IgG1, κ PE isotype control (bottom).
  • MP6-XT22_PE_TNF-a_Antibody_FC_1_100913
    PMA + Ionomycin-stimulated C57BL/6 mouse splenocytes (in the presence of monensin) were stained with CD3 APC, fixed, permeabilized and then stained with TNF-α (clone MP6-XT22) PE (top) or rat IgG1, κ PE isotype control (bottom).
  • MP6-XT22_PE_TNF-a_Antibody_FC_2_101413
See PE spectral data
Cat # Size Price Quantity Avail. Save
506305 25 µg $60.00
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506306 100 µg $200.00
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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse TNF-α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Detection: The biotinylated MP6-XT22 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 6B8 antibody (Cat. Nos. 510802 & 510804) as the capture antibody.
ELISA Capture: The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF-α (Cat. No. 575209) as the standard.
Flow Cytometry6,11,12:The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.
Neutralization1,5,10,16,17:The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF-α. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse TNF-α bioactivity in vivo and in vitro(Cat. No. 506310). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections7-9 in vivo detection5, immunofluorescence, and immunocytochemistry.
Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
  2. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20
  3. Mo X, et al. 1995. J. Virol. 69:1288.
  4. Sarawar S, et al. 1994. J. Immunol. 153:1246.
  5. Via C, et al. 2001. J. Immunol. 167:6821. (Neut)
  6. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (FC)
  7. Jacobs M, et al. 2000. Immunology 100:494. (IHC)
  8. Marinova-Mutachieva L, et al. 1997. Clin. Exp. Immunol. 107:507. (IHC)
  9. Williams RO, et al. 2000. J. Immunol. 165:7240. (IHC)
  10. Scanga CA, et al. 1999. Infect. Immun. 67:4531. (Neut)
  11. Akilov OE, et al. 2007. J. Leukoc. Biol. 2007;10.1189/jlb.0706439. (FC)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273. PubMed
  14. Wu S, et al. 2005. Neurosci Lett. 394:158. PubMed
  15. Carlson MJ, et al. 2009. Blood 113:1365. PubMed
  16. Shivakumar P, et al. 2017. JCI Insight. 2:e88747 1. PubMed
  17. Kearney CJ, et al. 2017. Cell Death Differ. 10.1038/cdd.2017.94. PubMed
Product Citations
  1. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  2. Chen D, et al. 2018. Nat Commun. 9:873. PubMed
  3. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  4. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  5. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  6. Stutz MD et al. 2018. Frontiers in immunology. 1.193055556 . PubMed
  7. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  8. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  9. Lu Y, et al. 2019. Int J Mol Med. 43:2398. PubMed
  10. Bennion BG, et al. 2019. J Virol. 93. PubMed
  11. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  12. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  13. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  14. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  15. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  16. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  17. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  18. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  19. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  20. Liu W, et al. 2020. Mol Ther Oncolytics. 17:350. PubMed
  21. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  22. Brenner E, et al. 2020. Nat Commun. 11:1335. PubMed
  23. Deczkowska A, et al. 2017. Nat Commun. 8:717. PubMed
  24. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  25. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  26. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
  27. Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
  28. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  29. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  30. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  31. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  32. Akilov O, et al. 2007. J Leukoc Biol. 81:1188. PubMed
  33. Dahlin J, et al. 2012. J Immunol. 189:3869. PubMed
  34. Asrat S, et al. 2014. PLoS Pathog. 10:1004229. PubMed
  35. Ni P, et al. 2014. Mol Immunol. 62:199. PubMed
  36. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  37. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  38. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  39. Huang J, et al. 2015. Biochem Biophys Res Commun. 463: 551-556. PubMed
  40. Cabrera-Mora M, et al. 2015. Infect Immun . 83: 3749-3761. PubMed
  41. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  42. Liu J, et al. 2016. Cancer Res . 76: 5288 - 5301. PubMed
  43. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  44. Knocke S, et al. 2016. Cell Rep. 17:2234-2246. PubMed
  45. Li M, et al. 2020. J Immunother Cancer. 8:00. PubMed
  46. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  47. C Khouili S, et al. 2020. Cell Rep. 33:108468. PubMed
  48. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  49. Fang Y, et al. 2021. J Clin Invest. 131:00:00. PubMed
  50. Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed
  51. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  52. Tello-Lafoz M, et al. 2021. Immunity. 54(5):1037-1054.e7. PubMed
  53. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  54. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  55. Xu S, et al. 2021. Immunity. . PubMed
RRID
AB_315426 (BioLegend Cat. No. 506305)
AB_315427 (BioLegend Cat. No. 506306)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 17.5-150 kD (Mammalian)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.

Regulation
Processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, and cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, and PAF antagonists
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10/14/2013

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