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PE anti-mouse TCR Vβ11 Antibody

Pricing & Availability
Clone
RR3-15 (See other available formats)
Regulatory Status
RUO
Other Names
TCR Vβ-11, V beta 11 TCR, Vβ11 T-cell receptor
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
RR3-15_PE_1_060710
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC and RR3-15 PE
  • RR3-15_PE_1_060710
    C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC and RR3-15 PE
  • RR3-15_PE_2_060710
    C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC and rat IgG2b, κ (RTK4530) PE isotype control
See PE spectral data
Cat # Size Price Quantity Check Availability Save
139003 25 µg $98
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139004 100 µg $214
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex.  The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.  V β 11-bearing T cells are largely absent from strains of mice that express MHC class II molecule I-E.  It has been demonstrated that the expression of I-E molecule is necessary for the clonal deletion of V beta 11-bearing T cells, but not sufficient for the clonal elimination of V beta 11-bearing T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse cytolytic T-cell clone OH6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: Western Blot1.

Application References

(PubMed link indicates BioLegend citation)
  1. Bill J, et al. 1989. J. Exp. Med. 169:1405. (WB)
Product Citations
  1. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  2. Meyers JL, et al. 2018. PLoS One. 13:e0207007. PubMed
RRID
AB_10613472 (BioLegend Cat. No. 139003)
AB_10612759 (BioLegend Cat. No. 139004)

Antigen Details

Structure
A member of Ig superfamily, heterodimerized with α TCR α chain, and associated with CD3 complex.
Distribution

Subsets of T cells and thymocytes.

Function
Antigen recognition.
Ligand/Receptor
Antigens
Cell Type
T cells, Thymocytes
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Kubo R, et al. 1989. J. Immunol. 142:2736.
3. Haqqi TM, et al. 1989. J. Exp. Med. 169:1903.
4. Behlke MA, et al. 1986. Proc. Natl. Acad. Sci. USA 83:767.
5. Gao EK, et al. 1989. J. Exp. Med. 170:1947.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR Vbeta11 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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