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- Clone
- MD-113 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MD1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse splenocytes stained with MD-113 PE
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 117805 | 50 µg | $214 | ||||
MD-1 is a small 28-kD molecule that is usually found in association with CD180 (RP105), a member of the Toll-like receptor family. MD-1 is primarily expressed by B cells, dendritic cells and monocytes, and promotes the recognition of, and subsequent signaling by, LPS in the innate immune system. The MD-113 antibody is reported to be antagonistic for LPS-induced B cell proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse RP105/MD-1 transfected NRK cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking of LPS-induced B cell proliferation1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 117808).
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Application References
(PubMed link indicates BioLegend citation) -
- Nagai Y, et al. 2002. Blood 99 :1699.
- RRID
-
AB_2137928 (BioLegend Cat. No. 117805)
Antigen Details
- Structure
- Acts in conjunction with the toll-like receptor family members, 28 kD
- Distribution
-
B cells, dendritic cells, and monocytes/macrophages
- Function
- Recognition and signaling in response to LPS
- Ligand/Receptor
- LPS
- Cell Type
- B cells, Monocytes, Macrophages, Dendritic cells
- Biology Area
- Immunology, Innate Immunity
- Antigen References
-
1. Nagai Y, et al. 2002. Blood 99 :1699.
- Gene ID
- 17084 View all products for this Gene ID
- UniProt
- View information about MD-1 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All MD-1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse MD-1 | MD-113 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse MD-1
C57BL/6 mouse splenocytes stained with MD-113 PE
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