PE anti-mouse Ki-67 Antibody

Pricing & Availability
Clone
16A8 (See other available formats)
Regulatory Status
RUO
Other Names
KIA, proliferation-related Ki-67 antigen
Isotype
Rat IgG2a, κ
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Product Citations
publications
16A8_PE_Ki67_Antibody_ICFC_082112
C57BL/6 mouse splenocytes were stimulated for 3 days with Con-A plus IL-2; subsequently, the cells were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 16A8_PE_Ki67_Antibody_ICFC_082112
    C57BL/6 mouse splenocytes were stimulated for 3 days with Con-A plus IL-2; subsequently, the cells were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
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652403 25 µg $153
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652404 100 µg $360
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed
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  3. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
  4. Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed
Product Citations
  1. Chan S, et al. 2022. Nat Cancer. . PubMed
  2. Liang J, et al. 2016. Nat Med. 22:1285-1293. PubMed
  3. Sizova O, et al. 2018. Mol Cancer Res. 16:1652. PubMed
  4. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  5. Liu J, et al. 2015. Sci Rep. 5: 17569. PubMed
  6. Wu M, et al. 2021. Nat Commun. 12:3500. PubMed
  7. Ferretti M, et al. 2016. Brain Behav Immun. 54: 211-225. PubMed
  8. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  9. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
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  11. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  12. Feng Y, et al. 2018. Kidney Dis (Basel). 4:95. PubMed
  13. Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
  14. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  15. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  16. Schneider MK, et al. 2018. Physiol Rep. 6:e13645. PubMed
  17. Giannini S, et al. 2020. Nat Commun. 0.705555556. PubMed
  18. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  19. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  20. Song J, et al. 2021. Cancer Commun (Lond). 41:867. PubMed
  21. Haas MS, et al. 2020. Mol Cancer Res. 19:717. PubMed
  22. Lee J, et al. 2020. Cell Reports. 33(13):108560. PubMed
  23. Godavarthy PS, et al. 2019. Haematologica. 104(10). PubMed
  24. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  25. Shani O, et al. 2021. Elife. 10: . PubMed
  26. Watson MJ, et al. 2021. Nature. 591:645. PubMed
  27. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  28. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  29. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  30. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  31. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
  32. Sun X, et al. 2021. Nature. 599:673. PubMed
  33. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  34. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  35. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  36. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
  37. Muzumdar S, et al. 2019. Am J Pathol. 189:568. PubMed
  38. Kim GC, et al. 2020. Front Oncol. 10:642. PubMed
  39. Fang W, et al. 2021. J Exp Clin Cancer Res. 40:4. PubMed
  40. Ghura H, et al. 2021. Neoplasia. 23:837. PubMed
  41. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  42. Zamarin D, et al. 2020. Clin Cancer Res. 26:4531. PubMed
  43. Lawson M, et al. 2015. Nat Commun. 6: 8983. PubMed
  44. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  45. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  46. Zhang Y, et al. 2021. JCI Insight. 6:e150735. PubMed
  47. Wang X, et al. 2022. Elife. 11:. PubMed
  48. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  49. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  50. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
  51. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  52. Oladiran O, et al. 2021. Front Immunol. 12:720733. PubMed
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  54. Nguyen HD, et al. 2020. Cell Rep. 33:108316. PubMed
RRID
AB_2561524 (BioLegend Cat. No. 652403)
AB_2561525 (BioLegend Cat. No. 652404)

Antigen Details

Structure
325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution

Nucleus and chromosome

Function
Required for cell cycle progression and proliferation
Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.

Gene ID
17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 01/08/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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