- Clone
- GL7 (See other available formats)
- Other Names
- Ly77, T and B cell activation marker
- Isotype
- Rat IgM, κ
- Ave. Rating
- 1 reviews
- Product Citations
- publications
-
BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and anti-mouse GL7 (clone GL7 PE (top) or rat IgM, κ PE isotype control (bottom). -
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 144607 | 25 µg | $85 | ||||
| 144608 | 100 µg | $215 | ||||
The GL7 antigen, also known as Ly77, is a 35 kD protein. The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes. It is upregulated on mouse splenocytes following activation. It may play a role in regulation or adhesion. GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.
Product DetailsProduct Details
- Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- LPS activated DBA/J mouse B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
The GL7 antibody does not block the binding of CD22 with sulfated α2-6-sialyl-LacNAc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
- Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
- Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
- Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
- Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
- Product Citations
-
- RRID
-
AB_2562925 (BioLegend Cat. No. 144607)
AB_2562926 (BioLegend Cat. No. 144608)
Antigen Details
- Structure
- 35 kD
- Distribution
-
Germinal center B cells, activated B and T cells
- Function
- Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
- Ligand Receptor
- Neu5Ac-recognizing lectins
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89. - Gene ID
- NA
- UniProt
- View information about GL7 on UniProt.org
Reviews
-
- Title
- Rating
- Date
-
08/01/2016
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.