- Clone
- GL7 (See other available formats)
- Other Names
- Ly77, T and B cell activation marker
- Isotype
- Rat IgM, κ
- Ave. Rating
- 1 reviews
- Product Citations
- publications
-
BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and anti-mouse GL7 (clone GL7 PE (top) or rat IgM, κ PE isotype control (bottom). -
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 144607 | 25 µg | $85.00 | ||||
| 144608 | 100 µg | $215.00 | ||||
The GL7 antigen, also known as Ly77, is a 35 kD protein. The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes. It is upregulated on mouse splenocytes following activation. It may play a role in regulation or adhesion. GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.
Product DetailsProduct Details
- Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- LPS activated DBA/J mouse B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
The GL7 antibody does not block the binding of CD22 with sulfated α2-6-sialyl-LacNAc.
-
Application References
(PubMed link indicates BioLegend citation) -
- Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
- Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
- Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
- Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
- Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
- Jutz S, et al. 2016. J. Immunol Methods. 430:10-20. PubMed
- Product Citations
-
- RRID
-
AB_2562925 (BioLegend Cat. No. 144607)
AB_2562926 (BioLegend Cat. No. 144608)
Antigen Details
- Structure
- 35 kD
- Distribution
- Germinal center B cells, activated B and T cells
- Function
- Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
- Ligand Receptor
- Neu5Ac-recognizing lectins
- Antigen References
-
1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89. - Gene ID
- NA
- UniProt
- View information about GL7 on UniProt.org
Reviews
-
- Title
- Rating
- Date
-
08/01/2016
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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