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PE anti-mouse F4/80 Recombinant Antibody

Pricing & Availability
Clone
QA17A29 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA17A29_PE_F4-80_Antibody_122319_updated.png
Thioglycollate-elicited Balb/c mouse peritoneal macrophages were stained with mouse F4/80 PE (clone QA17A29) (left) or isotype control PE (right) and mouse CD11b APC.
  • QA17A29_PE_F4-80_Antibody_122319_updated.png
    Thioglycollate-elicited Balb/c mouse peritoneal macrophages were stained with mouse F4/80 PE (clone QA17A29) (left) or isotype control PE (right) and mouse CD11b APC.
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157303 25 µg $44
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157304 100 µg $153
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  2. Gao Z, et al. 2022. J Inflamm Res. 15:3337. PubMed
RRID
AB_2832546 (BioLegend Cat. No. 157303)
AB_2832547 (BioLegend Cat. No. 157304)

Antigen Details

Structure
A 160 kD glycoprotein and a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family
Distribution

Expressed on the majority of tissue macrophages

Function
Required for induction of CD8+ T cells-mediated peripheral tolerance
Cell Type
Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035.
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3.
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354.
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077.
  5. Glass AM, et al. 2013. J. Immunol. 190:4830.
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570.
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526.
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. 
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322.
  10. Ohnishi A, et al. 2015. PLoS One. 10:117616.
Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12/23/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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