PE anti-mouse F4/80 Recombinant Antibody

Pricing & Availability
QA17A29 (See other available formats)
Regulatory Status
Other Names
EMR1, Ly71
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
Thioglycollate-elicited Balb/c mouse peritoneal macrophages were stained with mouse F4/80 PE (clone QA17A29) (left) or isotype control PE (right) and mouse CD11b APC.
  • QA17A29_PE_F4-80_Antibody_122319_updated.png
    Thioglycollate-elicited Balb/c mouse peritoneal macrophages were stained with mouse F4/80 PE (clone QA17A29) (left) or isotype control PE (right) and mouse CD11b APC.
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
157303 25 µg $44
Check Availability

Need larger quantities of this item?
Request Bulk Quote
157304 100 µg $153
Check Availability

Need larger quantities of this item?
Request Bulk Quote

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Murine macrophages
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  2. Gao Z, et al. 2022. J Inflamm Res. 15:3337. PubMed
AB_2832546 (BioLegend Cat. No. 157303)
AB_2832547 (BioLegend Cat. No. 157304)

Antigen Details

A 160 kD glycoprotein and a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family

Expressed on the majority of tissue macrophages

Required for induction of CD8+ T cells-mediated peripheral tolerance
Cell Type
Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035.
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3.
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354.
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077.
  5. Glass AM, et al. 2013. J. Immunol. 190:4830.
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570.
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526.
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. 
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322.
  10. Ohnishi A, et al. 2015. PLoS One. 10:117616.
Gene ID
13733 View all products for this Gene ID
View information about F4/80 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12/23/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse F4/80 Recombinant Antibody

  • PE anti-mouse F4/80 Recombinant Antibody

  • APC anti-mouse F4/80 Recombinant Antibody

  • PE/Cyanine7 anti-mouse F4/80 Recombinant Antibody

  • FITC anti-mouse F4/80 Recombinant Antibody

  • Spark YG™ 593 anti-mouse F4/80 Recombinant Antibody

  • APC/Cyanine7 anti-mouse F4/80 Recombinant Antibody

  • PerCP/Cyanine5.5 anti-mouse F4/80 Recombinant Antibody

  • Alexa Fluor® 647 anti-mouse F4/80 Recombinant Antibody

  • PE/Fire™ 640 anti-mouse F4/80 Recombinant Antibody


Remember me
Forgot your password? Reset Password
Request an Account