PE anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
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Product Citations
publications
16-10A1_PE_032309.jpg
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with 16-10A1 PE
  • 16-10A1_PE_032309.jpg
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with 16-10A1 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
104707 50 µg $90
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104708 200 µg $250
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Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Cross-Reactivity: Dog (Canine)
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  2. Zhang F, et al. 2017. Mol Immunol. 10.1016/j.molimm.2017.06.019. PubMed
  3. Ye Y, et al. 2017. Sci Immunol. 2:17. PubMed
  4. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
  5. Xie Z, et al. 2018. J Am Heart Assoc. 7:e007442. PubMed
  6. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  7. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  8. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  9. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  10. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  11. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  12. Lin SY, et al. 2017. Autophagy. 14:778. PubMed
  13. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  14. Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed
  15. Matundan H, et al. 2019. J Virol. 93. PubMed
  16. Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed
  17. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  18. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  19. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  20. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  21. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  22. Yang C, et al. 2019. Nanomedicine (Lond). 14:2423. PubMed
  23. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  24. He J, et al. 2020. Evid Based Complement Alternat Med. 2020:5637507. PubMed
  25. Lightman SM, et al. 2020. Cell Reports. 1.086805556. PubMed
  26. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  27. Rafiq S, et al. 2018. Nat Biotechnol. 36:847. PubMed
  28. Rozanski C, et al. 2011. J Exp Med. 208:1435. PubMed
  29. Simmons D, et al. 2012. J Immunol. 188:3116. PubMed
  30. Marshall N, et al. 2012. Cancer Res. 72:581. PubMed
  31. Jeisy-Scott V, et al. 2012. J Virol. 86:10988. PubMed
  32. Wong E, et al. 2012. J Immunol. 189:5667. PubMed
  33. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  34. White C, et al. 2015. J Immunol. 194:697. PubMed
  35. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  36. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  37. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
  38. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  39. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  40. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
RRID
AB_313128 (BioLegend Cat. No. 104707)
AB_313129 (BioLegend Cat. No. 104708)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/18/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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