PE anti-mouse CD366 (Tim-3) Antibody

Pricing & Availability
Clone
B8.2C12 (See other available formats)
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
B8dot2C12_PE_060309.jpg
Mouse CD366 (Tim-3) transfected cells stained with anti-mouse CD366 (Tim-3, clone B8.2C12) PE.
  • B8dot2C12_PE_060309.jpg
    Mouse CD366 (Tim-3) transfected cells stained with anti-mouse CD366 (Tim-3, clone B8.2C12) PE.
See PE spectral data
Cat # Size Price Quantity Avail. Save
134003 50 µg $110
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134004 200 µg $335
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mTim-3 protein/Freund adjuvant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone B8.2C12 only binds to the BALB/c allele of Tim-3.

Application References

(PubMed link indicates BioLegend citation)
  1. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Puleston D, et al. 2014. Elife. 3:3706. PubMed
  2. Zhu H, et al. 2018. Elife. 7:. PubMed
  3. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  4. Xie A et al. 2017. Endocrinology. 158(10):3140-3151 . PubMed
RRID
AB_1626181 (BioLegend Cat. No. 134003)
AB_1626177 (BioLegend Cat. No. 134004)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain. It can exist as a soluble form lacking mucin and transmembrane domains.
Distribution

Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.

Function
Play a role in regulating macrophage activation, T cell apoptosis and immune tolerance.
Ligand/Receptor
Galectin -9
Cell Type
Dendritic cells, Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454
3. Mooney L, et al. 2002. Nature. 415:536
4. Rodriguez-Manzanet R, et al. 2009. Immunol. Rev. 229(1):259

Gene ID
171285 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04/10/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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