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PE anti-mouse CD300c/d Antibody

Pricing & Availability
Clone
TX52 (See other available formats)
Regulatory Status
RUO
Other Names
CD300c/d, MAIR-II, LMIR-II, CLM-4, DIgR1, CLM-6
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
TX52_PE_CD300c-d_Antibody_FC_070814.jpg
CD300c/d/MAIR-II transfectants (ST88) cells were stained with anti-mouse CD300c/d (clone TX52) PE (filled histogram) or rat IgG2b PE isotype control.
  • TX52_PE_CD300c-d_Antibody_FC_070814.jpg
    CD300c/d/MAIR-II transfectants (ST88) cells were stained with anti-mouse CD300c/d (clone TX52) PE (filled histogram) or rat IgG2b PE isotype control.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
148003 25 µg $153
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148004 100 µg $347
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Description

CD300 receptors are a group of type I transmembrane proteins, which belong to the Ig superfamily. In mice, nine members have been reported with different nomenclature systems, such as myeloid-associated Ig-like receptor (MAIR), leukocyte mono-Ig-like receptor (LMIR), DC-derived Ig-like receptor (DIgR), and CMRF-35-like molecule (CLM). The CD300 molecules act as either activating or inhibitory receptors through interactions with adaptor molecules, such as PAD12, DAP10, and FcRγ. Despite sharing high homology with CD300a, CD300c (LMIR-II) does not share its inhibitory function. CD300c has been found to activate a variety of cell types and induce proinflammatory molecule production. CD300d may be involved with the regulation/formation of CD300 complexes on the cell surface.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
MAIR-II transfected Ba/F3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1.

RRID
AB_2563674 (BioLegend Cat. No. 148003)
AB_2563675 (BioLegend Cat. No. 148004)

Antigen Details

Structure
Type I transmemrane protein, Ig superfamily
Distribution
B cell subsets, macrophages, dendritic cells
Interaction
DAP12, DAP10, FcRγ
Ligand/Receptor
Phosphatidylserine
Cell Type
B cells, Macrophages, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Yotsumoto K, et al. 2003. J. Exp. Med. 198:223.
2. Martinez-Barriocanal A, et al. 2010. J. Biol. Chem. 285:41781.
3. Borrego F. 2013. Blood. 121:1951.
4. Lankry D, et al. 2013. Eur. J. Immunol. 43:2151.

Gene ID
387565 View all products for this Gene ID 140497 View all products for this Gene ID
UniProt
View information about CD300c/d on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 07/17/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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