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- Clone
- RTAA15 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B7H3, B7RP-2
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Thioglycolate-elicited BALB/c mouse peritoneal macrophages stimulated with LPS for 24 hours and then stained with RTAA15 PE
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 124507 | 25 µg | $92 | ||||
| 124508 | 100 µg | $275 | ||||
B7-H3 is a member of the B7 gene family. It has 88% amino acid sequence identity with human B7-H3. It is mostly expressed on professional APCs, including B cells, macrophages, and dendritic cells. Its expression on dendritic cells appears to be up-regulated by LPS. B7-H3 protein inhibits T cell activation and cytokine production. It was reported that monoclonal antibody against B7-H3 enhanced T cell proliferation in vitro and led to exacerbated EAE in vivo. Therefore, B7-H3 may be a negative regulator on T cell activation and function.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Xu J, et al. 2006. Cellular and Molecular Immunology. 3(3):235
- Prasad D.V.R. 2004. J.Immunology.173:2500
- Product Citations
-
- RRID
-
AB_1279208 (BioLegend Cat. No. 124507)
AB_1279206 (BioLegend Cat. No. 124508)
Antigen Details
- Structure
- About 49.7 kd transmembrane protein characterized by extracellular IgV and IgC domains.
- Distribution
-
Mouse B7-H3 is expressed on professional APCs, including B cells, dendritic cells, and macrophages. It is reported to express on a minor fraction of CD4+ and CD8+ cells.
- Ligand/Receptor
- The receptor has not been identified, but it seems to be expressed on activated T cells.
- Bioactivity
- Mouse B7-H3 is a negative regulator of T cell activation and IL-2 production.
- Cell Type
- Antigen-presenting cells, Dendritic cells
- Biology Area
- Immunology, Costimulatory Molecules
- Molecular Family
- CD Molecules
- Antigen References
-
1. Sun M et al. 2002. J. Immunology. 168:6294
2. Xu J et al. 2006. Cellular and Molecular Immunology. 3(3):235 - Gene ID
- 102657 View all products for this Gene ID
- UniProt
- View information about CD276 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD276 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse CD276 (B7-H3) | RTAA15 | FC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE anti-mouse CD276 (B7-H3)
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