PE anti-mouse CD20 Antibody

Pricing & Availability
Clone
SA271G2 (See other available formats)
Regulatory Status
RUO
Other Names
Ms4a1, Ly-44
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
SA271G2_PE_CD20_Antibody_121019.png
C57BL/6 mouse splenocytes were stained with CD45R/B220 APC/Cyanine7 and CD20 (clone SA271G2) PE (left) or rat IgG2b, κ PE isotype control (right).
  • SA271G2_PE_CD20_Antibody_121019.png
    C57BL/6 mouse splenocytes were stained with CD45R/B220 APC/Cyanine7 and CD20 (clone SA271G2) PE (left) or rat IgG2b, κ PE isotype control (right).
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152105 25 µg $153
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152106 100 µg $335
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Description

CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca2+ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD20 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Bongard, A, et al. 2019. PLoS Pathog. 15(9):e1008043. (Depletion) Pubmed
RRID
AB_2819901 (BioLegend Cat. No. 152105)
AB_2819902 (BioLegend Cat. No. 152106)

Antigen Details

Structure
Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
Distribution

B cells and a subset of T cells

Function
Regulates B-cell activation and proliferation
Interaction
Associates with MHC class I and II, CD53, CD81, and CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Morsy DE, et al. 2013. J. Immunol. 191:3112.
2. Lund FE, Randall TD. 2010. Nat. Rev. Immunol. 10:236.
3.Beers SA, et al. 2010. Blood 115:5191.
4. Kuijpers TW, et al. 2010. J. Clin. Invest. 120:214.

Gene ID
12482 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12/10/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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