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PE anti-mouse CD19 Recombinant Antibody

Pricing & Availability
Clone
QA17A27 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA17A27_PE_CD19_Antibody_082020
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε APC and anti-mouse CD19 PE (clone QA17A27) (left) or mouse IgG1, κ PE isotype control (right).
  • QA17A27_PE_CD19_Antibody_082020
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε APC and anti-mouse CD19 PE (clone QA17A27) (left) or mouse IgG1, κ PE isotype control (right).
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159803 25 µg $80
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159804 100 µg $214
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone QA17A27 of antibody can block binding of 6D5 clone on target and vice versa.

RRID
AB_2876558 (BioLegend Cat. No. 159803)
AB_2876558 (BioLegend Cat. No. 159804)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Interaction
B cells, Dendritic cells
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Fearon DT. 1993. Curr Opin Immunol. 5:341-8.
  2. Krop I, et al. 1996. Eur J Immunol. 26:23-42.
  3. Krop I, et al. 1996. J Immunol. 157:48-56.
  4. Tedder TF, et al. 1994. Immunol. Today 15:437-42.
Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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