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PE anti-mouse CD16.2 (FcγRIV) Antibody

Pricing & Availability
Clone
9E9 (See other available formats)
Regulatory Status
RUO
Other Names
FcgRIV, Fcgr4, Fcrl3, CD16-2, FcgRIV, Fcgr3a, FcgammaRIV, 4833442P21Rik, Fc gamma RIV
Isotype
Armenian Hamster IgG
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Product Citations
publications
9E9_PE_CD16.2_Antibody_FC_051815
C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E9) PE (filled histogram) or Armenian hamster IgG PE (open histogram).
  • 9E9_PE_CD16.2_Antibody_FC_051815
    C57BL/6 bone marrow cells were stained with CD16.2 (clone 9E9) PE (filled histogram) or Armenian hamster IgG PE (open histogram).
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149503 50 µg $98
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149504 200 µg $304
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Description

FcγRIV, also known as CD16.2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16.2 is the mouse homolog of human FcγRIIIA. CD16.2 is a low-affinity IgE receptor for all allotypes and the ligation of FcγRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
FCγR4 —EC domain fusion with IgG1 Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block)
  2. Nimmerjahn F, et al. 2005. Immunity 23:41.
Product Citations
  1. de Tymowski C et al. 2019. Cell reports. 27(3):762-775 . PubMed
  2. Chauhan P, et al. 2017. Sci Rep. 7:41889. PubMed
  3. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  4. Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed
  5. Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed
RRID
AB_2565810 (BioLegend Cat. No. 149503)
AB_2565811 (BioLegend Cat. No. 149504)

Antigen Details

Structure
22.4 kD
Distribution

Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine

Function
Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.
Ligand/Receptor
IgG2a and IgG2b
Cell Type
B cells, Leukocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Mechetina LV, et al. 2002. Immunogenetics. 54:463-8.
  2. Nimmerjahn F, et al. 2005. Immunity. 23:41-51.
  3. Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.
Gene ID
246256 View all products for this Gene ID
UniProt
View information about CD16.2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05/18/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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