PE anti-mouse CD133 Antibody

Pricing & Availability
Clone
315-2C11 (See other available formats)
Other Names
Prominin-1, AC133
Isotype
Rat IgG2a, λ
Ave. Rating
1 reviews
Product Citations
publications
315-2C11_PE_1_020811
Mouse CD133 transfected RBL-2H3 cells were stained with CD133 (clone 315-2C11) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 315-2C11_PE_1_020811
    Mouse CD133 transfected RBL-2H3 cells were stained with CD133 (clone 315-2C11) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 315-2C11_PE_2_050411
    C57BL/6 mouse bone marrow cells were stained with FITC-conjugated Ly-6G, CD3 and B220, CD117 APC and CD133 (clone 315-2C11) PE (middle) or rat IgG2a, κ PE (bottom) isotype control. Data was gated on Ly-6G/CD3/B220 negative cell population.
  • 315-2C11_PE_3_050411
See PE spectral data
Cat # Size Price Quantity Avail. Save
141203 25 µg $115
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141204 100 µg $295
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Description

CD133 (also known as prominin-1 and AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD133-expressing-GFP-RBL2H3 plus CFA as adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Zhang Y, et al. 2012. Diabetes. 61:2114. PubMed
Product Citations
  1. Liu W, et al. 2011. PLoS One. 6:e23311. PubMed
  2. Zhang Y, et al. 2012. Diabetes. 61:2114. PubMed
  3. Contador-Troca M, et al. 2013. Carcinogenesis. 34:2683. PubMed
  4. Morimoto-Suzki N, et al. 2014. Development. 141:4343. PubMed
  5. Kim S, et al. 2015. Cancer Lett . Available online 29 August 2015. PubMed
  6. Bhatwadekar A, et al. 2015. Diabetes. 64: 4226 - 4237. PubMed
  7. Wei X, Orjalo A, Xin L 2016. Stem Cell Res. 16: 597-606. PubMed
  8. Ge Q, et al. 2018. Mol Med Rep. 17:1667. PubMed
  9. Baron CS, et al. 2018. Nat Commun. 9:2517. PubMed
Publication Library
RRID
AB_10718631 (BioLegend Cat. No. 141203)
AB_10722606 (BioLegend Cat. No. 141204)

Antigen Details

Structure
120 kD pentaspan transmembrane glycoprotein
Distribution
Hematopoietic stem cells, epithelial progenitor cells, leukemia cells, brain tumor cells, kidney, pancreas, placenta, and fetal liver cells
Function
Biological function unknown
Cell Type
Hematopoietic stem and progenitors, Epithelial cells, Leukemia, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Singh SK, et al. 2004. Nature 18:396.
2. Falini B, et al. 2005. New Engl. J. Med. 352:254.
3. Bonanno G, et al. 2004. Transfusion 44:1087.
4. Gaipa G, et al. 2002. Haematologica 87:347.
5. Wuchter C, et al. 2001. Haematologica 86:154.

Gene ID
19126 View all products for this Gene ID
UniProt
View information about CD133 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04/22/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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