PE anti-human/mouse/rat MR1 Antibody

Pricing & Availability
Clone
26.5 (See other available formats)
Other Names
Major Histocompatibility Complex Class I-related, HLALS
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
26.5_PE_MR1_Antibody_FC_012114
Human MR1-transfected Hela cell line was stained with MR1 (clone 26.5) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • 26.5_PE_MR1_Antibody_FC_012114
    Human MR1-transfected Hela cell line was stained with MR1 (clone 26.5) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Avail. Save
361105 25 tests $125
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361106 100 tests $295
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Description

MHC-related protein 1, also known as MR1, is a nonclassical MHC class I molecule highly conserved in mammals, particularly in its α1/α2 domains. MR1 is required for the development and expansion of the mucosal-associated invariant T (MAIT) cells. The transcripts for MR1 are ubiquitously expressed, but its expression on the cell surface seems to be ligand-dependent. MR1 is involved in the presentation of vitamin-derived compounds from bacterial biosynthetic pathways.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, Cross-Reactivity: Bovine (Cow)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Soluble complexes of MR1 ectodomain and B2M.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for the relevant formats include: blocking MR1-dependent activation of MAIT hybridomas, clones, or MAIT TCR-transduced cell lines1-6, detection of folded MR1 conformers3,7, and immunoprecipitation of MR1 from cell lysates7.

Application References

(PubMed link indicates BioLegend citation)
  1. Young MH, et al. 2013. PLoS One. 8:e53789. (Block)
  2. Leeansyah E, et al. 2013. Blood 121:1124. (Block)
  3. Kjer-Nielsen L, et al. 2012. Nature 491:717. (Block, ELISA)
  4. Reantragoon R, et al. 2012. J. Exp. Med. 209:761. (Block, FC)
  5. Gold MC, et al. 2010. PLoS Biol. 8:e1000407. (Block, FC)
  6. Huang S, et al. 2009. Proc. Natl. Acad. Sci. USA 106:8290. (Block, FC)
  7. Huang S, et al. 2005. J. Biol. Chem. 280:21183. (IP, ELISA, FC)
Product Citations
  1. Laugel B, et al. 2016. J Immunol. 197: 971 - 982. PubMed
RRID
AB_2563042 (BioLegend Cat. No. 361105)
AB_2563043 (BioLegend Cat. No. 361106)

Antigen Details

Structure
Member of the MHC class I family, 1 Ig-like C1-type domain, forms heterodimer with B-2 Microglobulin.
Distribution

MR1 transcripts are ubiquitously expressed, but its expression in the cell surface seems to be ligand-dependent.

Function
Antigen presentation, required for development and expansion of the mucosal-associated invariant T (MAIT) cells.
Ligand/Receptor
α-mannosyl ceramide derivatives, 6-formyl pterin, derivatives from bacterial riboflavin biosynthetic pathway).
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Gold MC and Lewinsohn DM. 2013. Nat. Rev. Microbiol. 11:14.
2. Young MH and Gapin L. 2013. Cell Res. 23:460.
3. Gold MC, et al. 2013. Mucosal Immunol. 6:35.
4. Meierovics A, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E3119.

Gene ID
3140 View all products for this Gene ID 15064 View all products for this Gene ID 25119 View all products for this Gene ID
UniProt
View information about MR1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/22/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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