PE anti-human integrin β5 Antibody

Pricing & Availability
Clone
AST-3T (See other available formats)
Other Names
integrin b5, integrin beta 5, ITGB5
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
AST-3T_PE_110309
Human cervical cancer cell line HeLa stained with AST-3T PE.
  • AST-3T_PE_110309
    Human cervical cancer cell line HeLa stained with AST-3T PE.
See PE spectral data
Cat # Size Price Quantity Avail. Save
345203 25 tests $120
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Description

Integrins are a family of transmembrane proteins. A total 17 integrin α chains and 8 β chains have been identified. The association of integrin α and β chains forms 25 integrin complexes which serve as extracellular matrix receptors and play important roles in cell adhesion and development. Integrin β5 is a 95 kD glycoprotein heterodimer with the αV subunit and is found on many types of tissue cells, such as epithelial cells, endothelial cells, keratinocytes, and osteoblastic cells. The αV/β5 integrin complex binds to vitronectin.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Perna F, et al. 2017. Cancer Cell. 32:506. PubMed
  2. Zuidema A, et al. 2018. J Cell Sci. 131. PubMed
Publication Library
RRID
AB_2129167 (BioLegend Cat. No. 345203)

Antigen Details

Structure
Integrin family, Ig superfamily, associates with αV subunit
Distribution
Epithelial cells, endothelial cells, keratinocytes, osteoblastic cells
Function
Cell adhesion
Ligand/Receptor
vitronectin
Cell Type
Epithelial cells, Endothelial cells, Osteoclasts
Biology Area
Immunology
Molecular Family
Adhesion Molecules
Antigen References

1. Kim JP. et al. 1994. J. Bio. Chem. 269:26926
2. Cheng SL. et al. 2000. J. Cell Biochem. 77:265
3. Aplin JD. et al. 1996. Mol. Hum. Reprod. 2:527

Gene ID
3693 View all products for this Gene ID
UniProt
View information about Integrin beta 5 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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