PE anti-human Ikaros Antibody

Pricing & Availability
Clone
16B5C71 (See other available formats)
Regulatory Status
RUO
Other Names
IK1, IKZF1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
16B5C71_PE_Ikaros_Antibody_1_062816
Human peripheral blood mononuclear cells were stained with CD19 Brilliant Violet 421™, treated with the True-Nuclear™ Transcription Factor Buffer Set, and then stained with Ikaros (clone 16B5C71) PE (top) or mouse IgG2a, κ PE isotype control (bottom).
  • 16B5C71_PE_Ikaros_Antibody_1_062816
    Human peripheral blood mononuclear cells were stained with CD19 Brilliant Violet 421™, treated with the True-Nuclear™ Transcription Factor Buffer Set, and then stained with Ikaros (clone 16B5C71) PE (top) or mouse IgG2a, κ PE isotype control (bottom).
  • 16B5C71_PE_Ikaros_Antibody_2_062816
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
368409 25 tests $138
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368410 100 tests $330
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Description

Ikaros, also known as IKZF1, belongs to a family of zinc-finger DNA-binding proteins that regulate lymphocyte differentiation. It has the ability to form homo- and heterodimers with other members of the Ikaros family, which include Aiolos and Helios. Ikaros can be found on a majority of fetal and adult hematopoietic cell types such as T- and B-cells, natural killer cells (NKs), monocytes/macrophages, and dendritic cells. Ikaros is a tumor suppressor and its absence or reduced expression has been reported in several leukemias.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ikaros peptide (431-477 aa) conjugated to KLH Freund's adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

RRID
AB_2616977 (BioLegend Cat. No. 368409)
AB_2616978 (BioLegend Cat. No. 368410)

Antigen Details

Structure
Zinc-finger DNA binding protein, homo/heterodimer, 57.5 kD
Distribution

Fetal and adult hematopoietic cells.

Function
Regulates lymphocyte differentiation.
Interaction
DNA.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Wargnier A, et al. 1995. Proc. Natl. Acad. Sci. USA 92:6930.
2. Tonnelle C, et al. 2009. Mol. Immunol. 46:1736.
3. Wang H, et al. 2014. Pediatr. Blood Cancer 61:2230.

Gene ID
10320 View all products for this Gene ID
UniProt
View information about Ikaros on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06/28/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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