PE anti-human HLA-G Antibody

Pricing & Availability
87G (See other available formats)
Other Names
Human Leukocyte Antigen-G
Mouse IgG2a, κ
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Product Citations
Human cytotrophoblastic cell line (JEG-3) stained with 87G PE
  • 87G_PE_100908.jpg
    Human cytotrophoblastic cell line (JEG-3) stained with 87G PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
335905 25 tests $110
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335906 100 tests $250
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HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A,B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2(CD85j), ILT4(CD85d), and KIR2DL4(CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in thymus.

Product Details
Technical data sheet

Product Details

Human, African Green, Cynomolgus, Rhesus
Antibody Type
Host Species
HLA-G transfected cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

87G mAb reacts with isoforms of HLA-G1 and -G5. Additional reported applications (for the relevant formats) include: restoring HLA-G mediated suppression of allo-T cell proliferation and immunohistochemical staining of frozen tissue sections. Clone 87G is reported not to bind HLA-G in aldehyde fixed samples.4

Application References

(PubMed link indicates BioLegend citation)
  1. Odum N, et al. 1991. Eur. J. Immunol. 21:2121.
  2. Lila N, et al. 2001. P. Natl. Acad. Sci. USA 98:12150.
  3. Lila N, et al. 2002. Circulation. 105:1949.
  4. Blaschitz A, et al. 2000. Hum. Immunol. 61:1074.
  5. Balsamo M, et al. 2012. Eur J. Immunol. 42:1833. PubMed
Product Citations
  1. Li Y, et al. 2014. Am J Pathol. 184:3332. PubMed
  2. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  3. Villa–Alvarez M, et al. 2017. Oncoimmunology. 6:e1353856. PubMed
AB_1227710 (BioLegend Cat. No. 335905)
AB_1227709 (BioLegend Cat. No. 335906)

Antigen Details


Trophoblast cells, thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, various tumors

Inhibit NK and T cell-mediated cytolysis and T cell proliferation, induce apoptosis of activated CD8+ T cells, induce Th2 cytokine production
ILT2(CD85j), ILT4(CD85d), KIR2DL4(CD158d), CD8α/α
Cell Type
Dendritic cells, Epithelial cells, Macrophages, Monocytes
Biology Area
Molecular Family
MHC Antigens
Antigen References

1. Hunt JS, et al. 2005. FASEB J. 19:681.
2. Carosella ED, et al. 2008. Blood 111:4862.

Gene ID
3135 View all products for this Gene ID
View information about HLA-G on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/13/2013

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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