PE anti-human DcR1 (TRAIL-R3, CD263) Antibody

Pricing & Availability
Clone
DJR3 (See other available formats)
Other Names
TRAIL-R3, TRID, LIT, CD263, TNFRSF10C
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DJR3_PE_072007
Human peripheral blood granulocytes stained with DJR3 PE
  • DJR3_PE_072007
    Human peripheral blood granulocytes stained with DJR3 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
307005 25 tests $115
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307006 100 tests $250
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Description

DcR1 is a 65 kD GPI-anchored member of the TNFR superfamily (TNFRSF10C) also known as TRAIL-R3, TRID, CD263, and LIT. Although not expressed by most tumors, DcR1 is expressed at low levels on normal tissues, acting as a TRAIL decoy receptor, thus inhibiting apoptosis. DcR1 is termed a decoy receptor, as it does not have a functional death domain to induce apoptosis following TRAIL ligation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of DcR1-human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: The DJR3 antibody is useful for immunofluorescent staining and flow cytometric analysis of DcR1/TRAIL-R3 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307004) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Uno K, et al. 2003. Blood 101:3658.
  2. Sato K, et al. 2005. J. Immunol. 174:4025.
RRID
AB_2287506 (BioLegend Cat. No. 307005)
AB_2205089 (BioLegend Cat. No. 307006)

Antigen Details

Structure
TNFR superfamily, GPI-anchored, truncated death domain, 65 kD
Distribution

Most normal tissues, not tumors

Function
TRAIL decoy, inhibits apoptosis
Ligand Receptor
TRAIL
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Schneider P, et al. 1997. J. Biol. Chem. 272:25417.
2. Pan G, et al. 1997. Science 277:815.

Gene ID
8794 View all products for this Gene ID
UniProt
View information about CD263 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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