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PE anti-human CD86 Antibody

Pricing & Availability
Clone
IT2.2 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD86.8
Other Names
B7-2, B70
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
IT2.2
Human peripheral blood monocytes stained with IT2.2 PE
  • IT2.2
    Human peripheral blood monocytes stained with IT2.2 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
305405 25 tests $110
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305438 100 µg $292
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305406 100 tests $297
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, Western blotting3, and blocking of T cell activation2,4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Dieu M. 1998. J. Exp. Med. 188:373. (Block)
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
  5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
  6. Geissmann F, et al. 2001. Blood 97:1241. (IHC)
Product Citations
  1. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  2. Rampal R, Awasthi A, Ahuja V 2016. Sci Rep. 6:30277. PubMed
  3. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  4. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  5. Gu C, et al. 2019. J Immunol. 203:389. PubMed
  6. Casasola-LaMacchia A, et al. 2021. Sci Rep. 11:1028. PubMed
  7. Schimek V, et al. 2022. Cell Death Dis. 13:113. PubMed
  8. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
  9. Bosco M, et al. 2011. Blood. 117:2625. PubMed
  10. Philippeos C, et al. 2018. J Invest Dermatol. 138:811. PubMed
  11. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  12. Du X, et al. 2018. Cell Res. 28:416. PubMed
  13. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
  14. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  15. Wang X, et al. 2021. Front Physiol. 12:644903. PubMed
  16. Haruna M, et al. 2020. Biol Pharm Bull. 43:399. PubMed
  17. Jankeel A, et al. 2020. J Virol. :94. PubMed
  18. Hofbauer D, et al. 2021. Immunity. 54(8):1772-1787.e9. PubMed
  19. Oulès B, et al. 2020. Nat Commun. 3.977083333. PubMed
  20. Bryson BD, et al. 2019. Nat Commun. 10:2329. PubMed
  21. Stoeker L, et al. 2011. Clin Vaccine Immunol. 2.023611111. PubMed
  22. Mendoza-Cabrera MI, et al. 2019. Innate Immun. :1753425919864658. PubMed
  23. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  24. Blaszczak AM, et al. 2019. J Diabetes Res. 2019:8124563. PubMed
  25. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  26. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  27. Planès R, et al. 2014. J Virol. 88:6672. PubMed
  28. Donini M, et al. 2011. Prostate. 72:566. PubMed
  29. Fiola S, et al. 2010. J Immunol. 185:3620. PubMed
  30. Li X, et al. 2021. Cell Death Dis. 12:314. PubMed
  31. Seeger P, et al. 2014. J Immunol. 192:1241. PubMed
  32. Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed
  33. Que Y, et al. 2021. Front Immunol. 12:653030. PubMed
  34. Wang X, et al. 2012. J Immunol. 189:3092. PubMed
  35. Finotti G, et al. 2016. J Leukoc Biol. 99: 107 - 119. PubMed
  36. Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed
  37. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  38. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  39. Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed
  40. Wee Y, et al. 2016. Exp Biol Med. 241: 1217 - 1228. PubMed
RRID
AB_314525 (BioLegend Cat. No. 305405)
AB_2564164 (BioLegend Cat. No. 305438)
AB_314526 (BioLegend Cat. No. 305406)

Antigen Details

Structure
Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution

Monocytes/macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321.

Gene ID
942 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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