PE anti-human CD86 Antibody

Pricing & Availability
Clone
IT2.2 (See other available formats)
Workshop
VI CD86.8
Other Names
B7-2, B70
Isotype
Mouse IgG2b, κ
Ave. Rating
3 reviews
Product Citations
publications
IT2.2
Human peripheral blood monocytes stained with IT2.2 PE
  • IT2.2
    Human peripheral blood monocytes stained with IT2.2 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
305405 25 tests $90
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305438 100 µg $240
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305406 100 tests $250
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Capuchin Monkey, Common Marmoset, Cotton-topped Tamarin, Chimpanzee, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
µg sizes: 0.2 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, Western blotting3, and blocking of T cell activation2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 305410).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Dieu M. 1998. J. Exp. Med. 188:373. (Block)
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
  5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
  6. Geissmann F, et al. 2001. Blood 97:1241. (IHC)
Product Citations
  1. Fiola S, et al. 2010. J Immunol. 185:3620. PubMed
  2. Stoeker L, et al. 2011. Clin Vaccine Immunol. 2.023611111. PubMed
  3. Wang X, et al. 2012. J Immunol. 189:3092. PubMed
  4. Bosco M, et al. 2011. Blood. 117:2625. PubMed
  5. Donini M, et al. 2011. Prostate. 72:566. PubMed
  6. Seeger P, et al. 2014. J Immunol. 192:1241. PubMed
  7. Planès R, et al. 2014. J Virol. 88:6672. PubMed
  8. Wee Y, et al. 2016. Exp Biol Med. 241: 1217 - 1228. PubMed
  9. Finotti G, et al. 2016. J Leukoc Biol. 99: 107 - 119. PubMed
  10. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  11. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  12. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  13. Rampal R, Awasthi A, Ahuja V 2016. Sci Rep. 6:30277. PubMed
  14. Du X, et al. 2018. Cell Res. 28:416. PubMed
  15. Philippeos C, et al. 2018. J Invest Dermatol. 138:811. PubMed
  16. Lin JR 2018. eLife. 7: e31657. PubMed
Publication Library
RRID
AB_314525 (BioLegend Cat. No. 305405)
AB_2564164 (BioLegend Cat. No. 305438)
AB_314526 (BioLegend Cat. No. 305406)

Antigen Details

Structure
Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution

Monocytes/macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand Receptor
CD28, CD152
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321.

Gene ID
942 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

Reviews

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Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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