PE anti-human CD85h (ILT1) Antibody

Pricing & Availability
Clone
24 (See other available formats)
Regulatory Status
RUO
Other Names
ILT1, LIR-7, LILRA2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
24_PE_121508.jpg
Human peripheral blood monocytes stained with clone 24 PE
  • 24_PE_121508.jpg
    Human peripheral blood monocytes stained with clone 24 PE
See PE spectral data
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337904 100 tests $304
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Description

CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). It is composed of both activating and inhibitory isoforms. The activating subset of ILTs is characterized by containing short cytoplasmic domains and positively charged arginine residues, while the inhibitory isoforms display long cytoplasmic tails containing ITIM motifs. CD85h is a 69kD activating receptor, also known as ILT1, LIR-7, or LILRA2. ILT1 is found on the surface of monocytes/macrophages, dendritic cells, and at relatively low levels on granulocytes. It is also expression on a small subset of NK cells. ILT1 acts as an activating receptor through its association with Fc receptor γ chain.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunohistochemical staining of acetone-fixed frozen tissue sections, Costimulation and activation of myeloid cells skewing of dendritic cell differentiation

Application References

(PubMed link indicates BioLegend citation)
  1. Lee DJ, et al. 2007 J. Immunol. 179 8128.
RRID
AB_2249947 (BioLegend Cat. No. 337904)

Antigen Details

Structure
ILT/LIR family, Ig superfamily, 69 kD glycoprotein
Distribution

Monocytes/macrophages, dendritic cells, granulocytes (low), small subset of NK cells

Ligand/Receptor
Associate with Fc receptor γchain
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedla N, et al. 2003. Proc. Natl. Acad. Sci. 100:1174
2. Nakajima H, et al. 1999. J. Immunol. 162:5

Gene ID
11027 View all products for this Gene ID
UniProt
View information about CD85h on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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