PE anti-human CD73 (Ecto-5'-nucleotidase) Antibody

Pricing & Availability
Clone
AD2 (See other available formats)
Regulatory Status
RUO
Workshop
V B-CD73.3
Other Names
Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_AD2_PE_091009
Human peripheral blood lymphocytes stained with AD2 PE and CD3 APC
  • A_AD2_PE_091009
    Human peripheral blood lymphocytes stained with AD2 PE and CD3 APC
  • B_AD2_PE_091009
    Human peripheral blood lymphocytes stained with AD2 PE and CD19 APC
Compare all formats See PE spectral data
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344003 25 tests $129
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344004 100 tests $283
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Description

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include:immunofluorescence3.

Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1993. J. Immunol. 151:6933.
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations
  1. Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed
  2. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  3. Xin Y, et al. 2021. Stem Cell Res Ther. 49:12. PubMed
  4. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  5. Shi L, et al. 2021. Stem Cell Res Ther. 12:147. PubMed
  6. Huang X, et al. 2019. Oxid Med Cell Longev. 2019:1305049. PubMed
  7. Gao G, et al. 2022. Am J Cancer Res. 12:1934. PubMed
  8. Sung SE, et al. 2021. Int J Mol Sci. 22:. PubMed
  9. Ali S, et al. 2018. Purinergic Signal. 14:371. PubMed
  10. Saze Z, et al. 2013. Blood. 122:9. PubMed
  11. Sung SE, et al. 2021. Asian Spine J. Online ahead of print.. PubMed
  12. Zhang J, et al. 2022. Int J Mol Sci. 23:. PubMed
  13. Kicova M, et al. 2020. Neoplasma. 67:933. PubMed
  14. Jungwirth N et al. 2018. Journal of molecular neuroscience : MN. 65(1):60-73 . PubMed
  15. Stocco E, et al. 2020. Front Cell Dev Biol. 0.515972222. PubMed
  16. Zhang M, et al. 2013. Urology. 81:465. PubMed
  17. Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed
  18. Hasmim M, et al. 2022. Front Immunol. 13:982821. PubMed
  19. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  20. Blázquez-Prunera A, et al. 2017. Stem Cell Res Ther. 10.1186/s13287-017-0552-z. PubMed
  21. Kolodziej M, et al. 2019. Adipocyte. 0.509722222. PubMed
  22. Zhang Y, et al. 2019. Aging (Albany NY). 11:12641. PubMed
  23. de Sousa BM, et al. 2021. NPJ Regen Med. 6:80. PubMed
  24. Yoshida R, et al. 2022. Cancer Res. :. PubMed
  25. Peng H, et al. 2020. Ann Transl Med. 0.927777778. PubMed
  26. Ross NT, et al. 2020. Nature Chemical Biology. 16(1):50-59.. PubMed
  27. Sun T, et al. 2019. Haematologica. 105:661. PubMed
  28. Okamura G, et al. 2020. Int J Mol Sci. 22:00. PubMed
  29. Yao X, et al. 2022. Bioeng Transl Med. 7:e10250. PubMed
  30. He H, et al. 2019. J Cell Mol Med. 23:4139. PubMed
  31. Wang W, et al. 2022. Int J Mol Sci. 23:. PubMed
  32. Labedz-Maslowska A, et al. 2020. Int J Mol Sci. 21:. PubMed
  33. Cantoni C, et al. 2021. Oncoimmunology. 1879530:10. PubMed
  34. Vaka R, et al. 2022. Stem Cell Res Ther. 13:20. PubMed
  35. Schultheiß C, et al. 2020. Immunity. 53(2):442-455.e4.. PubMed
  36. Hong Y, et al. 2020. Aging Cell. 19:e13128. PubMed
  37. Dong N, et al. 2022. World J Stem Cells. 14:556. PubMed
  38. Lee EC, et al. 2020. Int J Mol Sci. 21:00. PubMed
  39. Chijimatsu R, et al. 2022. Regen Ther. 21:52. PubMed
  40. Bi R, et al. 2020. Osteoarthritis Cartilage. 28:842. PubMed
RRID
AB_1877224 (BioLegend Cat. No. 344003)
AB_2298698 (BioLegend Cat. No. 344004)

Antigen Details

Structure
GPI-linked 5'-nucleotidase, 70 kD
Distribution

Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells

Function
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04/09/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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