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PE anti-human CD51/61 Antibody

Pricing & Availability
Clone
23C6 (See other available formats)
Regulatory Status
RUO
Workshop
V S246
Other Names
Vitronectin receptor, Integrin αV/β3, ITGAV, ITGB3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
23C6_PE_071307
Human melanoma cell line M21 stained with 23C6 PE
  • 23C6_PE_071307
    Human melanoma cell line M21 stained with 23C6 PE
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304406 100 tests $262
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Description

CD51/CD61 is an integrin complex known as αVβ3. It is expressed at high levels on osteoclasts, endothelial cells, and melanoma cells and at low levels on platelets and macrophages. CD51 is a heterodimer composed of disulfide-linked 125 kD and 24 kD proteins. CD61 is also a member of the integrin family known as gpIIIa or β3 integrin. It is a 110 kD common β subunit of CD51/CD61 or CD41/CD61 complex. CD51/CD61, also known as the vitronectin receptor, mediates the binding of platelets to immobilized vitronectin without prior activation. Other ligands include RGD-containing proteins such as fibrinogen, fibronectin, von Willebrand factor (vWf), laminin, thrombospondin and the neural adhesion molecule L1. CD51/CD61 also mediates cell-cell adhesion via interaction with CD31. CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components. The 23C6 antibody has been reported to be useful for blocking studies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Chicken, Cynomolgus, Rabbit
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation5, immunohistochemical staining of acetone-fixed frozen tissue sections5, immunofluorescence microscopy5, and blocking of cell adhesion4,6. The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304414).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp WB, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Horton M, et al. 1991. Exp. Cell Res. 195:368.
  4. Takahashi R, et al. 1999. Blood 93:1951. (Block)
  5. Davies J, et al. 1989. J. Cell Biol. 109:1817. (IF, IHC, IP)
  6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709. (FC, Block)
  7. Barau A, et al. 2010. J. Ultrasound Med. 29:173. PubMed
RRID
AB_314442 (BioLegend Cat. No. 304406)

Antigen Details

Structure
Integrin, CD51/CD61 heterodimer, 24 kD
Distribution

Platelets, B subset, activated T cells, monocytes/macrophages, osteoblasts, endothelial cells, melanoma

Function
Adhesion, bone metabolism and apoptosis
Ligand/Receptor
Vitronectin, von Willebrand factor, fibrinogen, thrombospondin, osteopontin, denatured collagen
Cell Type
B cells, Endothelial cells, Macrophages, Monocytes, Osteoclasts, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Davies J, et al. 1989. J. Cell Biol. 109:1817.
2. Nesbitt S, et al. 1993. J. Biol. Chem. 268:16737.

Gene ID
3685 View all products for this Gene ID 3690 View all products for this Gene ID
UniProt
View information about CD51 CD61 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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