PE anti-human CD317 (BST2, Tetherin) Antibody

Pricing & Availability
Clone
RS38E (See other available formats)
Workshop
VIII
Other Names
BST2, Tetherin, HM1.24, Bone marrow stromal antigen 2
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
RS38E_PE_051010
Human peripheral blood monocytes stained with RS38E PE
  • RS38E_PE_051010
    Human peripheral blood monocytes stained with RS38E PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
348405 25 tests $125
Check Availability


Need larger quantities of this item?
Request Bulk Quote
348406 100 tests $295
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD317, also known as BST2, Tetherin, and HM1.24, is a type II transmembrane GPI-protein with a molecular weight of about 29-33 kD. It is an interferon-induced protein expressed on dendritic cells, plasma cells, B lymphoblast cells, monocytes, granulocytes, T cells, NK cells, stromal cells, and some non-hematopoietic cells. BST2 inhibits cytokine production through interaction with ILT7 (CD85g). It is also involved in the regulation of B cell growth. More importantly, BST2 has been found to restrict the release of a number of viruses from infected cells, including all tested retroviruses (such as HIV-1) and some arenaviruses and filoviruses. In HIV-1 studies, it has been reported that BST2 retains the nascent virons on the surface of infected cells by incorporation of the protein into HIV-1 particles. HIV-1 Vpu is able to induce BST2 degradation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Pigtail, Rhesus Macaque
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This product is manufactured and sold under a license and covered by a number of issued patents. Users who intend to file a patent relating to the use of this product, or who intend to develop therapeutic use of this antibody, are required to report to BioLegend in advance.

Application References

(PubMed link indicates BioLegend citation)
  1. Ishikawa J, et al. 1995. Genomics 26:527.
  2. Miyagi E, et al. 2011. J. Virol. 85:11981. PubMed
  3. Yokoyama T, et al. 2013. Int. J. Cancer. 132: 472. (FC) PubMed
Product Citations
  1. Lau D, et al. 2011. J Virol. 85:9834. PubMed
  2. Fukuma A, et al. 2013. PLoS One. 8:61530.. PubMed
  3. Thippeshappa R, et al. 2013. J Virol. 87:6678. PubMed
  4. Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed
  5. Martrus G, et al. 2016. J Virol. 90: 9018 - 9028. PubMed
  6. Ma T, et al. 2016. Sci Rep. 6:34752. PubMed
Publication Library
RRID
AB_10567247 (BioLegend Cat. No. 348405)
AB_10564402 (BioLegend Cat. No. 348406)

Antigen Details

Structure
Type II transmembrane, GPI-anchored protein, 29-33 kD
Distribution

Dendritic cells, plasma cells, B lymphoblast cells, monocytes, granulocytes, T cells, NK cells, stromal cells

Function
Regulate B cell growth, inhibit cytokine production, retain progeny virons on the surface of infected cells
Ligand Receptor
ILT7 (CD85g)
Cell Type
Dendritic cells, Plasma cells, B cells, Monocytes, Granulocytes, T cells, NK cells
Biology Area
Immunology, Innate Immunity, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sugamata OT, et al. 1999. Biochem. Bioph. Res. Co. 258:583.
2. Neil SJ, et al. 2008. Nature 451:425.
3. Fitzpatrick K, et al. 2010. PLoS Pathog. 6:e1000701.
4. Azuma KS, et al. 2008. Cancer Sci. 99:2461.
5. Cao W, et al. 2009. J. Exp. Med. 206:1603.
6. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers.

Gene ID
684 View all products for this Gene ID
UniProt
View information about CD317 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account