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PE anti-human CD272 (BTLA) Antibody

Pricing & Availability
Clone
MIH26 (See other available formats)
Regulatory Status
RUO
Other Names
BTLA, B and T lymphocyte attenuator
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A_MIH26_PE_031110
Human peripheral blood lymphocytes stained with CD3 (UCHT1) PerCP/Cy5.5 and MIH26 PE (top) or mouse IgG2a,k PE isotype control
  • A_MIH26_PE_031110
    Human peripheral blood lymphocytes stained with CD3 (UCHT1) PerCP/Cy5.5 and MIH26 PE (top) or mouse IgG2a,k PE isotype control
  • B_MIH26_PE_031110
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344505 25 tests $153
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344506 100 tests $396
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Description

B and T lymphocyte attenuator (BTLA) is an Ig superfamily coinhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand Herpes Virus Entry Mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significant up-regulated expression of BTLA compared with effector and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It has been reported that BTLA may regulate T cell function through binding to B7-H4.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human BTLA transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
 
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of T cell proliferation and cytokine production1. Clone MIH26 has agonistic activity on BTLA, resulting in the inhibition of activation.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for use in in vitro and in vivo biofunctional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Otsuki N, et al. 2006. Biochem. Bioph. Res. Co. 344:1121.
  2. Okano M, et al. 2008. Clin. Exp. Allergy 38:1891.
Product Citations
  1. Wei F, et al. 2013. Proc Natl Acad Sci U S A. 110:2480. PubMed
  2. Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
  3. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  4. Xu X, et al. 2021. Elife. 10:. PubMed
  5. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  6. Bziat V, et al. 2021. Cell. . PubMed
  7. Zhang S, et al. 2021. Nat Commun. 12:2877. PubMed
  8. Ye Z, et al. 2016. Sci Rep. 6:20401. PubMed
RRID
AB_2043945 (BioLegend Cat. No. 344505)
AB_2065761 (BioLegend Cat. No. 344506)

Antigen Details

Structure
Ig superfamily, with similar structure to CTLA-4 and PD-1
Distribution

B cells and T lymphocytes, NK cells and dendritic cells

Function
Negative regulation of T cell activation, proliferation and cytokine production
Ligand/Receptor
HVEM
Cell Type
B cells, Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Watanabe N, et al. 2003. Nat. Immunol. 4:670.
2. Sun Y, et al. 2009. J. Immunol. 183:1946.
3. Gonzalez LC, et al. 2005. P. Natl. Acad. Sci. USA 102:1116.

Gene ID
151888 View all products for this Gene ID
UniProt
View information about CD272 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • PerCP/Cyanine5.5 anti-human CD272 (BTLA)

  • APC/Cyanine7 anti-human CD272 (BTLA)

  • PE/Cyanine7 anti-human CD272 (BTLA)

  • Alexa Fluor® 647 anti-human CD272 (BTLA)

  • PE/Dazzle™ 594 anti-human CD272 (BTLA)

  • FITC anti-human CD272 (BTLA)

  • TotalSeq™-A0170 anti-human CD272 (BTLA)

  • TotalSeq™-C0170 anti-human CD272 (BTLA)

  • Ultra-LEAF™ Purified anti-human CD272 (BTLA)

  • PE/Cyanine5 anti-human CD272 (BTLA)

  • TotalSeq™-B0170 anti-human CD272 (BTLA)

  • TotalSeq™-D0170 anti-human CD272 (BTLA)

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