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PE anti-human CD215 (IL-15Rα) Antibody

Pricing & Availability
Clone
JM7A4 (See other available formats)
Regulatory Status
RUO
Other Names
IL-15Rα chain, IL-15R, IL-15Rα
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
JM7A4_PE_071608.jpg
IFN-γ + LPS-stimulated human peripheral blood monocytes stained with JM7A4 PE.
  • JM7A4_PE_071608.jpg
    IFN-γ + LPS-stimulated human peripheral blood monocytes stained with JM7A4 PE.
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330207 25 tests $159
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330208 100 tests $329
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Description

IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology to IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains. Similar to IL-2Rα, IL-15Rα is able to form a heterotrimer with their β chain (CD122) and common γ chain (γc, CD132). But unlike IL-2Rα subunit, IL-15Rα itself binds to IL-15 with equivalent high affinity compared with the heterotrimeric complex. IL-15Rα is expressed as soluble or transmembrane forms by variety cells, such as activated monocytes, dendritic cells, CD8+ memory T cells, NK or NKT cells. IL-15Rα by itself forms stable complexes with IL-15 on cell surface and presents IL-15 in trans to neighboring cells. IL-15, like IL-2, is a pleiotropic four-helix bundle cytokine and plays a key role in promoting T and B cell survival and proliferation, preferentially in memory CD8+ T cell proliferation, and NK/NKT cell development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Vamosi G, et al. 2004. P. Natl. Acad. Sci. USA. 101:11082.
  2. Dubois S, et al. 2002. Immunity 17:537.
  3. Veerman KM, et al. 2012. J. Immunol. 188:1638. (FC) PubMed
  4. Vujanovic L, et al. 2010. Blood. 116:575. (FC) PubMed
  5. Kitaya K, et al. 2013. Biol. Reprod. 89:70. (FC) PubMed
Product Citations
  1. Marra P, et al. 2014. Cancer Res. 74:4908. PubMed
  2. Vidard L, et al. 2019. J Immunol. 203:676. PubMed
  3. Vujanovic L, et al. 2010. Blood. 116:575. PubMed
  4. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
RRID
AB_2124602 (BioLegend Cat. No. 330207)
AB_2124601 (BioLegend Cat. No. 330208)

Antigen Details

Distribution

Dendritic cells, activated monocytes, memory CD8 T cells and NK/NKT cells

Function
Support T and B cell proliferation and development, preferentially for memory CD8+ T cell proliferation, and NK/NKT cell development
Ligand/Receptor
IL-15 with high affinity
Cell Type
Dendritic cells, Monocytes, NK cells, NKT cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Giri JG, et al. 1995. EMBO J. 14:3654.
2. Giron-Michel J, et al. 2005. Blood 106:2302.
3. Lorenzen I, et al. 2005. J. Biol. Chem. 281:6642.
4. Sato N, et al. 2007. Proc. Natl. Acad. Sci. USA. 104:588.

Gene ID
3601 View all products for this Gene ID
UniProt
View information about CD215 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 06/02/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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