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PE anti-human CD207 (Langerin) Antibody

Pricing & Availability
Clone
10E2 (See other available formats)
Regulatory Status
RUO
Other Names
CLEC4K
Isotype
Mouse IgG1, κ
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Product Citations
publications
10E2_PE_1_060311
Monocyte-derived Langerhans cells were stimulated with recombinant human GM-CSF, TGF-β and IL-4 for 3 days, followed by an additional 3-day culture with rhGM-CSF and TGF-β. They were then stained with CD1a APC and CD207 (clone 10E2) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 10E2_PE_1_060311
    Monocyte-derived Langerhans cells were stimulated with recombinant human GM-CSF, TGF-β and IL-4 for 3 days, followed by an additional 3-day culture with rhGM-CSF and TGF-β. They were then stained with CD1a APC and CD207 (clone 10E2) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • 10E2_PE_2_060311
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352203 25 tests $134
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352204 100 tests $317
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Description

CD207, also known as Langerin, is a 40 kD type II transmemberane cell glycoprotein which belongs to C-type lectin with mannose binding specificity. It is predominantly expressed on Langerhans cells and induces the formation of Birbeck granules, the Langerhans cell hallmark organelle. It is also found on several other subtypes of dendritic cells, such as dermal CD103-positive dendritic cells and splenic CD8-positive dendritic cells. Langerin is generally thought to be involved in antigen processing. Recently, it has been found that HIV captured by Langerin was internalized into Birbeck granule and degraded, which results in inhibition of HIV-1 infection and subsequent transmission.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Primary human Langerhans cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported application (for the relevant formats) includes: blocking the binding of HIV-1 to Langerhans cells1.

Application References

(PubMed link indicates BioLegend citation)
  1. Witte LD, et al. 2007. Nat. Med. 13:367. (Block)
Product Citations
  1. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  2. Cavrois M, et al. 2019. PLoS One. 14:e0221181. PubMed
RRID
AB_10900993 (BioLegend Cat. No. 352203)
AB_10897451 (BioLegend Cat. No. 352204)

Antigen Details

Structure
40 kD glycoprotein
Distribution

Langerhans cells, dermal CD103-positive dendritic cells, and splenic CD8-positive dendritic cells

Function
Binding and processing antigen; capturing, internalizing and degrading HIV
Ligand/Receptor
Antigen containing mannose, N-acetyl glucosamine, and fucose
Cell Type
Dendritic cells, Langerhans cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Valladeau J, et al. 2000. Immunity 12:71.
2. Mc Dermott R, et al. 2002. Mol. Biol. Cell. 13:317.
3. Mizumoto N, et al. 2004. J. Clin. Invest. 113:701.
4. Witte LD, et al. 2007. Nat. Med. 13:367.

Gene ID
50489 View all products for this Gene ID
UniProt
View information about CD207 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04/19/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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