PE anti-human CD1a Antibody

Pricing & Availability
Clone
HI149 (See other available formats)
Workshop
V CD01.01
Other Names
T6, R4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HI149
Molt-4 (T leukemia cell line) stained with HI149 PE
  • HI149
    Molt-4 (T leukemia cell line) stained with HI149 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
300105 25 tests $90
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300106 100 tests $200
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Description

CD1a is a 49 kD member of the immunoglobulin superfamily also known as T6 and R4. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. CD1a plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1a has been implicated in thymic T cell development.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Patton KM, et al. 2005. Infect. Immun.73:2083. PubMed
  4. Curti A, et al. 2010. Haematologica. 95:2022. PubMed
Product Citations
  1. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  2. Schrøder M, et al. 2016. PLoS One. 11: 0157387. PubMed
  3. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  4. Lin JR 2018. eLife. 7: e31657. PubMed
Publication Library
RRID
AB_314019 (BioLegend Cat. No. 300105)
AB_314020 (BioLegend Cat. No. 300106)

Antigen Details

Structure
Ig superfamily, MHC I-like molecule, type I transmembrane glycoprotein, 49 kD
Distribution

Cortical thymocytes, Langerhans cells, dendritic cells

Function
Antigen presentation, lymphocyte activation, thymic T cell development
Ligand Receptor
CD1-restricted TCRs
Cell Type
Dendritic cells, Langerhans cells, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Blumberg RS, et al. 1995. Immunol. Rev. 147:5.
2. Calabi F, et al. 1991. Tissue Antigens 37:1.
3. Melian A, et al. 1996. Curr. Opin. Immunol. 8:82.

Gene ID
909 View all products for this Gene ID
UniProt
View information about CD1a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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