PE anti-human CD163 Antibody

Pricing & Availability
RM3/1 (See other available formats)
Regulatory Status
Other Names
GHI/61, M130, RM3/1, p155, Hemoglobin/haptoglobin complex receptor, Macrophage-associated antigen, ED2(rat), Macrophage marker
Mouse IgG1, κ
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Product Citations
IL-10 stimulated human peripheral blood monocytes stained with RM3/1 PE
  • RM3slash1_PE_122007
    IL-10 stimulated human peripheral blood monocytes stained with RM3/1 PE
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326505 25 tests $122
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326506 100 tests $268
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CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kuffer cells, monocytes, subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammation diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Human monocytes
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RM3/1 binds to domain 9 of CD163. Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Högger P, et al. 1998. J. Immunol. 161:1883. (FC)
  2. Zwadlo G, et al. 1987. Exp. Cell Biol. 55:295. (FC)
  3. Buechler C, et al. 2000. J. Leukoc. Biol. 67:97. (FC)
  4. Puig-Kroger A, et al. 2009. Cancer Res. 69:9395. (FC) PubMed
  5. Madsen M, et al. 2004. J. Biol. Chem. 279:51561. (FC)
  6. Jones K, et al. 2013. Clin Cancer Res. 19:731. (FC) PubMed
  7. Stewart DA, et al. 2012. Mol. Cancer Res. 10:727. (IF)
Product Citations
  1. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
  2. Cui L, et al. 2021. Am J Reprod Immunol. 86:e13436. PubMed
  3. Bryson BD, et al. 2019. Nat Commun. 10:2329. PubMed
  4. Chen Y, et al. 2021. Transl Lung Cancer Res. 10:2193. PubMed
  5. Chiang N et al. 2018. Cell chemical biology. 26(2):244-254 . PubMed
  6. Suenaga G, et al. 2016. PLoS One. 11:e0163944. PubMed
AB_893269 (BioLegend Cat. No. 326505)
AB_893265 (BioLegend Cat. No. 326506)

Antigen Details

134 kD (non-reduced)/155 kD (reduced) glycoprotein, Scavenger receptor superfamily
Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells
Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Haptoglobin-hemoglobin complex, TWEAK
Cell Type
Monocytes, Macrophages, Dendritic cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Roth J, et al. 1994. Transolantation. 57:127.
2. Van den Heuvel MM, et al.1999. J. Leukoc. Biol. 66:858.
3. Sulahian TH, et al. 2000. Cytokines 12:1312.
4. Fabriek BO, et al. 2007. J. Neuroimmunol. 187:179.

Gene ID
9332 View all products for this Gene ID
View information about CD163 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 05/01/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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