PE anti-human CD133 Antibody

Pricing & Availability
clone 7 (See other available formats)
Regulatory Status
Other Names
Prominin-1, AC133 Antigen, PROM1, PROML1
Mouse IgG1, κ
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Product Citations
Human peripheral blood mononuclear cells were stained with CD34 FITC and CD133 (clone clone 7) PE (top) or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the lymphocyte population.
  • clone7_PE_CD133_Antibody_1_121716
    Human peripheral blood mononuclear cells were stained with CD34 FITC and CD133 (clone clone 7) PE (top) or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the lymphocyte population.
  • Clone7_PE_CD133_Antibody_2_121516
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Cat # Size Price Quantity Check Availability Save
372803 25 tests $153
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372804 100 tests $372
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CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant partial human CD133 protein.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone can block the staining of AC133 clone in flow cytometry.

Application References

(PubMed link indicates BioLegend citation)
  1. Swaminathan SK, et al. 2010. J. Immunol. Methods 361:110.
Product Citations
  1. Xin Y, et al. 2021. Stem Cell Res Ther. 49:12. PubMed
  2. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  3. Deshmukh A, et al. 2018. Cancers (Basel). 10. PubMed
  4. Wan J, et al. 2021. Front Pharmacol. 11:590723. PubMed
  5. Wang HC, et al. 2022. J Biomed Sci. 29:99. PubMed
  6. Chang S, et al. 2020. Cell Death Dis. 0.869444444. PubMed
  7. Sahin ID, et al. 2020. Sci Rep. 10:11814. PubMed
  8. Duan X, et al. 2022. Cell Biosci. 12:60. PubMed
  9. Hinz S, et al. 2021. iScience. 24:103026. PubMed
  10. Wu S, et al. 2020. Exp Ther Med. 1.988888889. PubMed
  11. Saga R, et al. 2019. J Radiat Res. 60:298. PubMed
  12. Ho CM, et al. 2022. Am J Cancer Res. 12:1686. PubMed
  13. Shalabi SF, et al. 2021. Nat Aging. 1:838. PubMed
  14. King P, et al. 2021. Int J Oncol. 59:. PubMed
  15. Huang R, et al. 2020. Cell Death Dis. 11:234. PubMed
  16. Xuan Z, et al. 2020. Am J Cancer Res. 0.458333333. PubMed
  17. Hirao A, et al. 2021. Cancers (Basel). 13:. PubMed
AB_2632879 (BioLegend Cat. No. 372803)
AB_2632880 (BioLegend Cat. No. 372804)

Antigen Details

120 kD pentaspan transmembrane glycoprotein.

Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells

Cell Type
Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References
  1. Yin AH, et al. 1997. Blood. 90:5002.
  2. Miraglia S, et al. 1997. Blood. 90:5013.
  3. Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25. 
Gene ID
8842 View all products for this Gene ID
View information about CD133 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12/19/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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