PE anti-human CD11a Antibody

Pricing & Availability
Clone
HI111 (See other available formats)
Workshop
IV N231
Other Names
LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HI111
Human peripheral blood lymphocytes stained with HI111 PE
  • HI111
    Human peripheral blood lymphocytes stained with HI111 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
301207 25 tests $90
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301208 100 tests $200
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Description

CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cattle (Bovine, Cow), Capuchin monkey, Chimpanzee, Cynomolgus, Dog (Canine), Horse (Equine), Rabbit (Lapine), Sheep (Ovine)
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting2, and blocking of cell-cell interaction and inhibition the binding of ICAM-14. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301214).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press New York.
  2. Leite F, et al. 2002. Infec. Immun. 70:4336.
  3. Jiang Y, et al. 2005. Clin. Hemorheol. Microcircul. 32:261.
  4. BTchard D, et al. 2001. J. Immunol. 167:3099.
  5. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
  6. Choi EY, et al. 2008. Blood 111:3607. PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Ma Q, et al. 2002. J. Biol. Chem. 277:10638.
Product Citations
  1. Kachlany M 2014. Microbiology. 160:2443. PubMed
  2. Nicol B,et al. 2017. J Autoimmun. . 10.1016/j.jaut.2017.10.005. PubMed
  3. Lin JR 2018. eLife. 7: e31657. PubMed
Publication Library
RRID
AB_314145 (BioLegend Cat. No. 301207)
AB_314146 (BioLegend Cat. No. 301208)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β2 (CD18) forms LFA-1, 170-180 kD
Distribution

Leukocytes

Function
Adhesion, costimulation
Ligand Receptor
ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
Cell Type
Leukocytes, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146.

Gene ID
3683 View all products for this Gene ID
UniProt
View information about CD11a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 5    Revision Date: 07/13/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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