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PE anti-human CD112 (Nectin-2) Antibody

Pricing & Availability
Clone
TX31 (See other available formats)
Regulatory Status
RUO
Other Names
Poliovirus Receptor Related 2 Protein (PRR2), Nectin-2, Hve B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TX31_PE_110308.jpg
KG1a (human myeloid leukemia cell line) stained with TX31 PE
  • TX31_PE_110308.jpg
    KG1a (human myeloid leukemia cell line) stained with TX31 PE
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Cat # Size Price Quantity Check Availability Save
337409 25 tests $117
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337410 100 tests $317
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Description

CD112, known as poliovirus receptor related 2 protein (PRR2), Nectin-2, or Hve B, is a type I transmembrane glycoprotein, a member of the Ig gene superfamily. It is primarily found on myelomonocytic cells, megakaryocytes, dendritic cells, mast cells, CD34 positive stem cells, endothelial cells, epithelial cells, and neuronal cells. CD112 functions as a receptor for α-herpes viruses HSV-1 and pseudorabies virus (PRV). CD112 is an adhesion molecule involved in the formation of cell junction and regulation of NK- and T cell-mediated cytotoxicity through interaction with CD226 (DNAM-1) and other nectin family molecules.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: partially block CD226-Fc binding to CD112 transfectant cells and partially inhibit the cytotoxicity mediated by NK and T cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Tahara-Hanaoka S, et al.. 2004. Int. Immunol. 16:533
  2. Lim O, et al. 2013. PLoS One. 8:e53611. PubMed
  3. Gillory LA, 2013. PLoS One. 8:77753. PubMed
Product Citations
  1. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  2. Cordeau M, et al. 2016. PLoS One. 11:e0164401. PubMed
  3. Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed
  4. Grandclaudon M, et al. 2019. Cell. 179:432. PubMed
  5. Lim O, et al. 2013. PLoS One. 8:e53611. PubMed
  6. Kaito Y, et al. 2022. Oncol Lett. 23:51. PubMed
  7. Gillory L, et al. 2013. PLoS One. 8:77753. PubMed
  8. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  9. Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
  10. Siew Y, et al. 2015. Int Immunol. 27: 621 - 632. PubMed
RRID
AB_2174163 (BioLegend Cat. No. 337409)
AB_2269088 (BioLegend Cat. No. 337410)

Antigen Details

Structure
Type I transmembrane glycoprotein, Ig superfamily
Distribution

Myelomonocytic cells, megakaryocytes, dendritic cells, mast cells, CD34-positive stem cells, endothelial cells, epithelial cells, and neuronal cells.

Function
Adhesion, receptor for some Herpes Viruses
Ligand/Receptor
CD226, CD155, Nectin-1, -3, and -4, Herpes Viruses
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Mast cells, Megakaryocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication
2. Bachelet I, et al. 2006. J. Bio. Chem. 281:27190
3. Pende D, et al. 2006. Blood 107:2030
4. Bottino C, et al. 2003. J. Exp. Med. 198:557

Gene ID
5819 View all products for this Gene ID
UniProt
View information about CD112 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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