PE anti-HA.11 Epitope Tag Antibody

Pricing & Availability
Clone
16B12 (See other available formats)
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
16B12_PE_HApoint11_EpitopeTag_Antibody_080216
CHO-K1 cells (open histogram) or HA tag stably transfected cells (filled histogram) were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with HA.11 Epitope Tag (clone 16B12) PE.
  • 16B12_PE_HApoint11_EpitopeTag_Antibody_080216
    CHO-K1 cells (open histogram) or HA tag stably transfected cells (filled histogram) were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with HA.11 Epitope Tag (clone 16B12) PE.
See PE spectral data
Cat # Size Price Quantity Avail. Save
901517 25 µg $155
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901518 100 µg $385
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical data sheet

Product Details

Reactivity
YPYDVPDYA Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This antibody is effective in immunoblotting (WB), immunofluorescence (IF), and immunoprecipitation (IP) of tagged proteins.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
  1. Pecot MY, Malhotra V. 2004. Cell. 116:99.
  2. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
  3. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
  4. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
  5. Kolodziej P, Young R. 1991. Meth Enzymol. 194:508. (General)
  6. Field J, et al. 1988. Mol Cell Biol. 8:2159. (General)
  7. Majeed SR, et al. 2014. Nat Commun. 23:5891. (IF) PubMed
  8. Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
  9. Hatanaka H, et al. 2009. J. Biol. Chem. 23:15448. (WB) PubMed
  10. Zhang Y, et al. 2007. J. Biol. Chem. 282:13022. (WB) PubMed
  11. Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed
RRID
AB_2629622 (BioLegend Cat. No. 901517)
AB_2629623 (BioLegend Cat. No. 901518)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/04/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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