- 16B12 (See other available formats)
- Other Names
- HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
- Mouse IgG1, κ
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- Product Citations
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The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.Product Details
- YPYDVPDYA Tag
- Antibody Type
- Host Species
- Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
This antibody is effective in immunoblotting (WB), immunofluorescence (IF), and immunoprecipitation (IP) of tagged proteins.
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
(PubMed link indicates BioLegend citation)
- Pecot MY, Malhotra V. 2004. Cell. 116:99.
- Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
- Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
- Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
- Kolodziej P, Young R. 1991. Meth Enzymol. 194:508. (General)
- Field J, et al. 1988. Mol Cell Biol. 8:2159. (General)
- Majeed SR, et al. 2014. Nat Commun. 23:5891. (IF) PubMed
- Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
- Hatanaka H, et al. 2009. J. Biol. Chem. 23:15448. (WB) PubMed
- Zhang Y, et al. 2007. J. Biol. Chem. 282:13022. (WB) PubMed
- Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed
AB_2629622 (BioLegend Cat. No. 901517)
AB_2629623 (BioLegend Cat. No. 901518)
- Biology Area
- Cell Biology
- Gene ID
- View information about HA.11 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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